Poly-D lysine-coated PhenoPlate™ 96-well microplates (formerly named CellCarrier Ultra microplates) are coated with a synthetic polypeptide.
With black well walls and an optically-clear cyclic olefin bottom, poly-D lysine-coated PhenoPlate 96-well microplates are ideal for cell culture and imaging with high-content screening systems.
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These PhenoPlate 96-well microplates (formerly named CellCarrier Ultra microplates) for high content imaging applications are poly-D-lysine (PDL)-coated and are available in black in a 96-well format. The microplates benefit from a film bottom made of cyclic olefin, a plastic that has glass-like optical properties, providing superior image quality and also good cell adherence. They have an ultra-low plate bottom (0.2mm) for better well access when using water immersion and high NA objectives, and a new design, low profile polystyrene lid for reduced evaporation.
Poly-lysine is a synthetic positively-charged polymer, existing as two enantiomers: Poly-D-lysine (PDL) and Poly-L-lysine (PLL). Adherence of certain cell types to poly-lysine-coated surfaces is based on the electrostatic interaction of the poly-D-lysine polycation with the negative charges of the cell membrane. Use of poly-lysine coatings on plate surfaces can help mediate the negative charges of the cell membrane and the negative charge of the surface. Both PDL and PLL are commonly used however PDL is not degraded by cellular proteases and is therefore often the preferred choice. As Poly-lysine is a synthetic protein, it does not influence the signaling pathways of the cells and is completely free of any animal contaminants. Almost all cell types will adhere to Poly-lysine coated plate bottoms.
Well format: 96-well
|Number of Rows||8|
|Number of Columns||12|
|Well volume||425 µL|
|Recommended working volume||Up to 300 µL|
|Well diameter Top (mm)||6.84|
|Well diameter Bottom (mm)||6.4|
|Well depth (mm)||12.7|
|A1 to top offset (mm)||11.24|
|A1 to side offset (mm)||14.38|
|Well-to-well spacing (mm)||9|
|Coating Treatment||Poly-D lysine|
|Color||Black with optically-clear film bottom|
|Detection Method||Confocal Imaging, Fluorescence, High Content Imaging|
|Product Brand Name||PhenoPlate (formerly CellCarrier Ultra)|
|Unit Size||Case of 160|
|Wells Number||96 Wells|
From September 15, 2021 CellCarrier Ultra microplates are renamed PhenoPlate microplates. Questions you may have this change can be found in this document.
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Technical Note for PhenoPlate-384 Ultra Microplate