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PhenoPlate 96-well, black, optically clear flat-bottom, poly-D-lysine-coated, lids, case of 160

Poly-D lysine-coated PhenoPlate 96-well microplates (formerly named CellCarrier Ultra microplates) are coated with a synthetic polypeptide.

With black well walls and an optically-clear cyclic olefin bottom, poly-D lysine-coated PhenoPlate 96-well microplates are ideal for cell culture and imaging with high-content screening systems.

Part Number
Unit Size
List Price
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Quantity
6055500
Case of 40
1060.00 USD
 
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6055508
Case of 160
3824.00 USD
 
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Overview

These PhenoPlate 96-well microplates (formerly named CellCarrier Ultra microplates) for high content imaging applications are poly-D-lysine (PDL)-coated and are available in black in a 96-well format. The microplates benefit from a film bottom made of cyclic olefin, a plastic that has glass-like optical properties, providing superior image quality and also good cell adherence. They have an ultra-low plate bottom (0.2mm) for better well access when using water immersion and high NA objectives, and a new design, low profile polystyrene lid for reduced evaporation.

Poly-lysine is a synthetic positively-charged polymer, existing as two enantiomers: Poly-D-lysine (PDL) and Poly-L-lysine (PLL). Adherence of certain cell types to poly-lysine-coated surfaces is based on the electrostatic interaction of the poly-D-lysine polycation with the negative charges of the cell membrane. Use of poly-lysine coatings on plate surfaces can help mediate the negative charges of the cell membrane and the negative charge of the surface. Both PDL and PLL are commonly used however PDL is not degraded by cellular proteases and is therefore often the preferred choice. As Poly-lysine is a synthetic protein, it does not influence the signaling pathways of the cells and is completely free of any animal contaminants. Almost all cell types will adhere to Poly-lysine coated plate bottoms.

Packaging Notes:

  • Cases of 40 are packaged as 2 sleeves of 20 plates, lids included
  • Cases of 160 are packaged as 8 sleeves of 20 plates, lids included

Well Plate Dimensions

Well format: 96-well

DescriptionSpecification
Number of Rows8
Number of Columns12
Well volume425 µL
Recommended working volumeUp to 300 µL
Height (mm)14.35
Length (mm)127.76
Width (mm)85.48
Well diameter Top (mm)6.84
Well diameter Bottom (mm)6.4
Well depth (mm)12.7
A1 to top offset (mm)11.24
A1 to side offset (mm)14.38
Well-to-well spacing (mm)9

Specifications

Automation Compatible Yes
Coating Treatment Poly-D lysine
Color Black with optically-clear film bottom
Detection Method Confocal Imaging, Fluorescence, High Content Imaging
Material Cyclic Olefin
Product Brand Name PhenoPlate (formerly CellCarrier Ultra)
Shipping Condition Ambient
Sterile Aseptic
Unit Size Case of 160
Wells Number 96 Wells
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CellCarrier Ultra microplates are now named PhenoPlates

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