384-well poly-D-lysine coated (PDL-coated) microplates with black well walls and an optically-clear cyclic olefin bottom for high content analysis, high content screening, and other cellular assays.
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These CellCarrier Ultra microplates for high content imaging applications are PDL-coated and are available in black in a 384-well format. The CellCarrier Ultra HCS microplates benefit from a film bottom made of cyclic olefin, a plastic that has glass-like optical properties, providing superior image quality and also good cell adherence. They have an ultra-low plate bottom (0.2mm) for better well access when using water immersion and high NA objectives, and a new design, low profile polystyrene lid for reduced evaporation. Case of 160.
Poly-lysine is a synthetic positively-charged polymer, existing as two enantiomers: Poly-D-lysine (PDL) and Poly-L-lysine (PLL). Adherence of certain cell types to poly-lysine-coated surfaces is based on the electrostatic interaction of the poly-D-lysine polycation with the negative charges of the cell membrane. Use of poly-lysine coatings on plate surfaces can help mediate the negative charges of the cell membrane and the negative charge of the surface. Both PDL and PLL are commonly used however PDL is not degraded by cellular proteases and is therefore often the preferred choice. As Poly-lysine is a synthetic protein, it does not influence the signaling pathways of the cells and is completely free of any animal contaminants. Almost all cell types will adhere to Poly-lysine coated plate bottoms.
|Assay Technology||HCA ImagAmp|
|Detection Method||Confocal Imaging, High Content Imaging|
|Product Brand Name||CellCarrier|
|Unit Size||Case of 160|
|Wells Number||384 well plate|
Technical Note for CellCarrier-384 Ultra Microplate: Dedicated Design for High Content Screening