The Opera Phenix® Plus High Content Screening System is the premier confocal solution for today's most demanding high content applications. Drawing on over a decade of experience with the industry-leading Opera® and Opera® Phenix system, the Opera Phenix Plus is designed for high-throughput high-content assays, phenotypic screening, assays using complex disease models, such as live cells, primary cells and microtissues, and fast-response assays, such as Ca2+ flux.
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Deeper Insights From Your Most Demanding Assays
The Opera Phenix Plus high-content screening system’s innovative optical design lets you generate richer information through extremely sensitive confocal imaging and at higher throughput than ever through simultaneous acquisition. Because spectral crosstalk is reduced to a minimum, it delivers speed without compromising sensitivity. With proven automated water immersion lenses, you’ll achieve higher throughput and richer content, making it the ideal high-content screening system for discriminating phenotypes and studying complex disease models. And with its liquid handling option and fast imaging frame rate, you can tackle fast-response assays such as calcium flux or cardiomyocyte beating.
Speed or Sensitivity – No Compromise
Proprietary Synchrony™ Optics combine a microlens enhanced Nipkow spinning disk with dual view confocal optics to separate fluorescence excitation and emission during simultaneous acquisition minimizing spectral crosstalk – for greater speed and higher sensitivity.
Capture Fast Cellular Responses
The system’s imaging frame rate of up to 100fps is well-suited to assays that measure the beat-rate of cultured cardiomyocytes, as often used in cardiotoxicity studies. And the fast imaging frame rate, together with the dispense and read capabilities of the optional pipettor module, enables fast response assays in which cell responses occur within milliseconds to seconds – notably calcium flux assays.
It All Comes Together in Harmony® – Acquisition to Analysis Made Easy
Your Total Solution for High Content Screening
Extracellular signal-regulated kinase (ERK) is a key component in the regulation of embryogenesis, cell differentiation, cell proliferation, and cell death. The ERK signaling pathway is altered in various cancer types and is frequently investigated as a target for therapeutic intervention. This application note describes how a live cell FRET assay to study ERK signaling was performed on the Operetta CLS™ high-content analysis system. The optimized design of the FRET-based biosensor, the high-quality imaging of the Operetta CLS system and the easy-to-use image analysis tools of the Harmony® software contribute to the robustness of the high-content assay.
In this application note, we describe a high-content screening application for analyzing the migration of non-small cell lung cancer cells in a live cell assay. Using the Operetta® high-content imaging system and digital phase contrast imaging, we tracked migrating cancer cells using automated single cell tracking in the Harmony® high-content imaging and analysis software.
Cell Painting is a powerful high-content screening method which combines cell and computational biology to unravel cells’ responses and gain a deeper understanding of the effects of chemical and genetic perturbagens.
However, implementation of cell painting is not without its challenges - from choosing a cell model and labeling reagents, to optimizing instrumentation and making sense of the thousands of features that are extracted during data analysis.
Download our application note to learn how to:
The promise of high-content screening is the acceleration of discovery by extracting as much relevant information as possible from cells. Nevertheless, a large percentage of high-content screens analyze only a small number of image-based properties. As a result, valuable information from precious cells and disease models is not utilized. As nearly all screening approaches require a nuclear counterstain such as Hoechst to facilitate segmentation, phenotypic profiling of the nuclei can offer new and additional perspectives on assays at no extra cost.
Dysregulation of GPCR signaling, particularly calcium signaling, has been implicated in different diseases, e.g. cancer and Alzheimer’s disease. Currently about 34% of all FDA approved drugs target GPCRs indicating their importance for drug discovery, and in vitro cellular GPCR assays are important tools to identify new drugs.
Our application note describes how to analyze fast changes in intracellular calcium levels upon GPCR stimulation and inhibition using a fluorescence imaging assay on the Opera Phenix® Plus high-content screening system.
Analyzing transport of biliary metabolites is essential to predict pharmacokinetics and hepatotoxicity during drug development. A functional impairment of hepatobilary transporters, such as bile salt export pump (BSEP) and multidrug resistance-associated protein 2 (MRP-2), is strongly associated with an increased risk of cholestatic liver injury. Here, we describe a 3D high-content screening assay to study hepatobiliary transporter function in InSphero human liver microtissues. Confocal imaging and automated image analysis were used to quantify BSEP and MRP-2-mediated efflux of fluorescent substrates into bile canaliculi.
Fundamental processes in living cells, such as apoptosis and signal transduction are controlled by proteins, often acting in concert with other protein partners through protein-protein interactions (PPIs). Inappropriate protein-protein recognition can fundamentally contribute to many diseases, including cancer. Therefore, inhibiting protein-protein interactions represents an emerging area in drug design.
Learn how a phenotypic screening assay to study time-dependent effects of endothelin-1-induced hypertrophy was set up using human induced pluripotent stem cell (hiPSC)-derived cardiomyocytes. Learn how: The Opera Phenix system has been applied in the field of neurodegenerative diseases. In this assay, the Opera Phenix system is 4 times faster than the previous Opera® system. Primary neuron morphology is analyzed in a straightforward approach using Harmony software. Careful assay optimization can increase throughput, and minimize the data burden, without compromising assay performance.
Whether you’re familiar with high-content screening and are looking to exploit the increased physiological relevance of complex 3D cell models, or you want to take your analysis of 3D cell models to the next level, migrating from simple plate-reader assays to a high-content approach, you’ll need the right tools and strategies to overcome the challenges these models present.
The incidence and prevalence of non-alcoholic fatty liver disease (NAFLD), which is characterized by excessive accumulation of lipids within the liver, is high and progressively rising due to increasing obesity and metabolic syndrome prevalence. For some patients, NAFLD can progress to non-alcoholic steatohepatitis (NASH), which can lead to liver cirrhosis and hepatocellular carcinoma (HCC). However, NASH is poorly understood and therapies to treat the disease are lacking. Michele Vacca, from the University of Cambridge in the UK and colleagues sought to investigate the impact of one member of the TGFß-BMP superfamily, BMP8B, on the progression of NASH.
Within diabetes research, an evolving area of interest is the identification and development of an efficacious treatment for ß cell loss. The following literature review discusses the use of a Disque platform for evaluating the functionality of multiple drug candidates.
Live-cell imaging, the study of living cells using microscopy, has become a requisite technology in many fields of biomedical research, such as cell biology, developmental biology and cancer research. Also, in drug discovery, researchers adopt live-cell imaging as they look for a more detailed understanding of cellular behavior.
In our article, learn about:
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the virus that causes COVID-19, the respiratory illness responsible for the COVID-19 pandemic. Studies have shown that patients infected with SARS-CoV-2 with cardiovascular comorbidities are more susceptible to severe infection and thus the case fatality rate in these patients rises. To further understand the pathology of SARS-CoV-2 in cardiomyocytes, a team of researchers from the University of Cambridge in the UK, have developed a screening platform using human embryonic stem cell-derived cardiomyocytes (hESC-CMs).
Download our brochure to learn how our solutions help you to grow, detect, and analyze 3D cells.
Find out about our range of integrated solutions for drug discovery screening in this e-brochure.
Our screening solutions for high-throughput screening, phenotypic screening and data analysis help to streamline drug discovery workflows in labs across the globe. Our portfolio includes automated liquid handling, assays and reagents, imaging and detection systems, and informatics.
Working independently or together, with our solutions you can achieve consistent and accurate results. By accelerating the identification and characterization of effective and safe drug candidates, the PerkinElmer portfolio enables you to optimize efficiency in your lab and deliver more actionable, real-world results.
Download the brochure to learn more about how we can partner with you so that you can discover smarter, more effective, data-driven breakthroughs in the critical screening stages of drug discovery and development.
This case study illustrates how PerkinElmer’s high-content imaging solutions are enabling therapeutic antibody development. Two steps in the workflow - the clonal B cell selection and the functional antibody characterization - rely on high-content screening using the Operetta® system.
The coupling of the Operetta system to a plate::handler workstation allows the running of plates both day and night, further increasing the throughput. In addition, the EnVision multimode plate reader and the JANUS Automated Workstation are essential parts of the workflow that contribute to the throughput of the process and reduce the variability of liquid handling steps.
This case study shows how a previously-described neuroprotection assay was easily and directly transferred to the Opera Phenix® high-content screening system, with a 4-fold decrease in acquisition time. In the assay, primary rat neurons are co-cultured on top of rat-derived astrocytes. To induce axon degeneration experimentally, NGF is withdrawn, leading to neuronal cell death, while astrocytes remain healthy. This can be captured by two readouts: the total axon area and the total number of nuclei. Neuroprotective drug candidates lead to an increase in the total axon area while keeping the number of nuclei (astrocytes) constant. Decreasing nuclei counts indicate a cytotoxic effect.
Download the case study to learn how:
Antimicrobial resistance (AMR) is one of today’s major global public health challenges. Recently, there has been renewed investment in the discovery of novel antimicrobials to urgently address the growing number of drug-resistant infections. This case study describes how Professor Gordon Dougan and his team at the Cambridge Biomedical Research Centre are using high-content analysis to phenotype individual bacteria within a population to investigate adaptive mechanisms of antimicrobial resistance, and to screen for novel alternatives to existing antimicrobials.
Download this booklet from The Scientist and PerkinElmer to learn about how the third dimension affects cell behavior, the similarities and differences between 2-D and 3-D culture, common 3-D culture models, and how to image and analyze 3-D culture models.
While 3D cell culture provides unprecedented opportunities for both increased physiological relevance and analysis using a high-content approach, it is also more complex than traditional 2D cell culture. This booklet, from Biocompare and PerkinElmer, will unravel some of the complexities often encountered when using 3D cell models for drug discovery and provide insights and solutions that will streamline workflows and facilitate the development of effective therapeutics. Topics covered include: Reagents and instruments for growing, detecting, and analyzing 3D cell models; 3D culture methodologies; the value of high-content screening with 3D cell models and how to improve image acquisition and image analysis with high-content assays.
Infectious diseases remain a major burden to human health. The increased globalization of modern society that facilitates the spread of infectious diseases, and phenomena such as anti-microbial resistance, underscore the importance of the development of new preventative and therapeutic approaches.
Download this booklet to learn how high-content imaging and analysis enable high-throughput functional and phenotypic assays that can be adapted to a wide range of pathogens; read a series of Featured Publication Notes describing the contribution of high-content analysis in the study of diseases such as ebola, zika, tuberculosis, listeria and malaria and find examples of studies in which a high-content approach has been used in parasitic, viral and bacterial disease research.
Please download the pdf and view in Adobe Reader or Acrobat for optimum performance.
Live-cell imaging has evolved from allowing observation of large-scale changes to capturing subtle changes in dynamic cellular processes. Today, live-cell imaging coupled with high-content analysis enables researchers to extract quantitative data in real-time, facilitating new insights in basic life science research and drug discovery. Modern live-cell imaging systems can capture rapid cellular events, track cell movement, monitor protein signaling, screen cell health, and much more.
Download the eBook from The Scientist and PerkinElmer to discover:
Whether you’re familiar with high-content screening, or a newcomer, you’ll need the right tools and strategies to overcome the challenges of using complex 3D cell models in such an assay. For example, growing consistent, reproducible 3D cultures can be problematic and imaging large, thick cell samples can be challenging, while managing the huge volumes of data generated is perhaps the most demanding aspect of all. In this article, we provide our top tips for running a successful high-content screening assay using a 3D cell model. Learn how you can: Generate uniform 3D cell models, Get the best quality images, Minimize imaging time and volume of data, Get deeper insights from your 3D cell model and Avoid unnecessary data transfer steps.
High-content assays using 3D objects such as cysts or organoids can be challenging from the perspectives of both image acquisition and image analysis. In this technical note we describe how to image and analyze epithelial Madin-Darby canine kidney (MDCK) cysts in 3D on the Operetta CLS™ high-content analysis system.
Multicellular 3D “oids” (tumoroids, spheroids, organoids) have the potential to better predict the effects of drug candidates during preclinical screening. However, compared to 2D cell monolayers, assays using 3D model systems are more challenging.
In this technical note we describe how to image and analyze solid spheroids in 3D using the Opera Phenix™and Operetta™CLS high-content screening systems and Harmony® 4.8 imaging and analysis software.
In drug discovery programs, multicellular spheroids have emerged as powerful tools to bridge the gap between in vitro cell culture models and in vivo tissues. However, one of the greatest challenges in higher throughput 3D imaging is the acquisition of images of solid spheroids, owing to the reduced light penetration.
One solution is to use optical clearing techniques, which can enhance the imaging depth in spheroids by removing lipid and protein molecules.
In this technical note, we compare different optical clearing strategies for 3D spheroids and quantify the clearing effectiveness and alterations in spheroid morphology, and demonstrate how to increase imaging depth in 3D spheroids by a factor of four.
Live cell imaging has gained importance within drug discovery over recent times, as researchers look for more meaningful insights into cellular behavior and function. However, setting up live cell experiments can be challenging, as temperature, CO2 and evaporation need to be controlled to ensure optimal cell growth conditions. In this technical note, we demonstrate:
Download our technical note to find out how you can overcome some of the challenges associated with long-term live cell imaging. Learn how you can perform successful five-day live cell imaging on Operetta CLS™ and Opera Phenix™ high-content systems, avoid phototoxicity with gentle digital phase contrast imaging, and analyze cell growth and morphology on a single cell level without fluorescence staining.
Automated image acquisition and analysis of tissue sections can be challenging owing to the inherent height variations throughout each specimen, and this can hinder their use in screening applications.
In this technical note we show how PreciScan™ intelligent image acquisition enables generation of accurate high-resolution images, overcoming issues caused by factors such as height variations in the specimen or varying positions of the tissue sections on the slides, while also significantly reducing the data volume and acquisition time.
The Opera Phenix™ high-content screening system’s state of the art hardware, combined with Harmony® imaging and analysis software, improves acquisition and analysis speed, enabling users to perform highly multi-parametric phenotypic screens effectively. In this technical note, learn how the Opera Phenix system’s Synchrony™ Optics and sCMOS cameras image up to 6x faster, how water immersion objectives combined with binning can reduce exposure time by approx. 20-fold and how image acquisition and analysis time can be reduced by >70% compared to a classical spinning disc confocal system.
Simultaneous multi-color imaging is a technology commonly used to increase the speed of high content screening systems. However, one of the main problems arising from this approach is spectral crosstalk which can limit assay sensitivity. To overcome this challenge, we have developed an innovative optical concept for the Opera Phenix™ High Content Screening System, known as Synchrony Optics™. Learn more in our Technical Note.
Spinning disk confocal microscopy is a common tool for live cell microscopy and reduces background fluorescence from out of focus planes. However, for 3D imaging, confocal image quality can be limited by a phenomenon known as ‘pinhole crosstalk’ which can lead to blurred images in conventional spinning disk systems. The confocal scanning unit of the Opera Phenix™ High-Content Screening System provides an increased distance between pinholes to reduce the pinhole crosstalk, leading to improved 3D image quality. Learn more in our Technical Note.
The key parameters in high-content imaging - speed, sensitivity (or intensity) and resolution - cannot be optimized independently as altering one of them influences the others. For example, increasing the resolution by choosing a higher magnification requires imaging of more fields to maintain the same cell number – at the expense of speed; or increasing the fluorescence intensity can be achieved by increasing the exposure time – again at the expense of speed. Nevertheless, there is a way to overcome some of these obstacles and here, in this Technical Note, we explain why the choice of the objective lens is critical.
There has been a lot of buzz around artificial intelligence, machine learning and deep learning. Is the reality living up to the hype?
In the world of cellular imaging and its application to drug discovery, there is evidence of real progress against some of the critical challenges facing scientists using these technologies.
In this white paper, you will learn about:
Phenotypic Drug Discovery Re-Imagined
Cell Painting is a phenotypic screening method and a powerful application of high-content screening technology which combines cell and computational biology to elucidate the behavior of cells under the influence of perturbagens, such as chemical compounds, drugs, genes, or other entities.
Download our white paper to learn about the origins of the Cell Painting technique, its potential impact on the drug discovery paradigm, together with practical hints and tips for success.
Researchers are increasingly looking to 3D cell cultures, microtissues, and organoids to bridge the gap between 2D cell cultures and in vivo animal models. This whitepaper documents a streamlined procedure for getting the most information, as quickly as possible, using solutions from PerkinElmer.
Today's drug discovery strategies require candidate compounds to fail early and cheaply in the discovery stage, rather than late and expensively in the clinical phase. Testing compounds early in physiologically relevant model systems and leveraging rich information from image-based screens are ways of focusing on those compounds that give rise to the right phenotypic changes without undesirable effects on the system.