PerkinElmer
check quantity

Opal Polaris 7 Color Manual IHC Detection Kit

Introducing Phenoptics 2.0: Opal Polaris Kits are part of a practical workflow for the simultaneous detection of up to six tissue biomarkers plus nuclear counterstain within a single image, across the entire digital slide. The method is similar to standard immunohistochemistry (IHC) and is accessible to many laboratories where standard IHC method development is performed. Opal Polaris 7-Color IHC Detection Kits are optimized for whole slide multispectral imaging with the Vectra® Polaris Automated Quantitative Pathology Imaging Instrument.

You successfully added item(s) to your cart

For research use only. Not for use in diagnostic procedures.

Part Number
List Price
Your Price
Quantity
NEL861001KT
1792.00 USD
more
Buy Now

Please enter valid quantity

Please login to add favorites

NULL OR EMPTY CART

Detail Information

The Opal Polaris 7-Color Manual IHC Detection Kit is part of the Phenoptics 2.0 whole slide workflow and contains:

  • 6 reactive fluorophores (Opal Polaris 480, Opal 520, Opal 570, Opal 620, Opal 690, and Opal Polaris 780)
  • Spectral DAPI
  • 1 X Plus Amplification Diluent
  • Blocking/Ab Diluent
  • Opal Polymer HRP Ms + Rb
  • AR6 Buffer
  • Users should source primary antibodies and other workflow reagents separately. The Opal 7-Color Manual IHC Kit was optimized for use on the Vectra® Polaris system. Kits contain enough reagents to stain 50-slides at the recommended 1:100 dilution ratio.

What is Opal? Opal Multiplex IHC Kits make multiplex methods accessible to researchers who works with standard immunohistochemistry (IHC). The Opal method allows use of any number of unlabeled primary antibodies from the same species in multiplexed tissue assays, with no fear of cross-reactivity. Antibodies for simultaneous IHC may now be selected based on performance, rather than species. The method works with formalin fixed paraffin-embedded (FFPE) tissue and is compatible with the standard research IHC workflow in your lab.

Opal provides researchers with the tools to interrogate multiple pathways while retaining context provided by tissue images. This approach provides information not available from alternative techniques like flow cytometry or analysis of single markers in serial sections.

Opal will enable you to:

  • Measure up to six tissue biomarkers at once
  • Use the best primary antibodies together in multiplex panel, with no species-based crosstalk
  • Retain spatial cellular context that is lost in flow cytometry
  • Confirm single cell co-expression for many biomarkers in one tissue section
  • Get more information while conserving precious tissue.

Specifications

Automation Compatible Yes
Detection Method MSI, Fluorescence
Fluorescent Agent Type Opal Polaris 480, Opal 520, Opal 570, Opal 620, Opal 690, and Opal Polaris 780
Product Brand Name Opal
Quantity in a Package Amount 1.0 per pack
Sample Type Human FFPE
Shipping Condition Blue Ice
Species Reactivity Rabbit and Mouse Primary Antibodies
Unit Size 50 slides
Resources, Events & More
  • All

Brochure

Data Sheet

Product Note

Product Note: Mantra Quantitative Pathology Workstation

The Mantra™ quantitative pathology workstation, with inForm® image analysis software, enables easy visualization, quantification, and phenotyping of multiple types of immune cells simultaneously in intact FFPE tissue sections for cancer immunology research.

PDF 4 MB