Introducing Phenoptics 2.0™: Opal™ Polaris Kits are part of a practical workflow for the simultaneous detection of up to six tissue biomarkers plus nuclear counterstain within a single image, across the entire digital slide. The method is similar to standard immunohistochemistry (IHC) and is accessible to many laboratories where standard IHC method development is performed. Opal Polaris 7-Color IHC Detection Kits are optimized for whole slide multispectral imaging with the Vectra® Polaris™ Automated Quantitative Pathology Imaging Instrument.
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The Opal Polaris 7-Color Manual IHC Detection Kit is part of the Phenoptics 2.0 whole slide workflow and contains:
What is Opal? Opal Multiplex IHC Kits make multiplex methods accessible to researchers who works with standard immunohistochemistry (IHC). The Opal method allows use of any number of unlabeled primary antibodies from the same species in multiplexed tissue assays, with no fear of cross-reactivity. Antibodies for simultaneous IHC may now be selected based on performance, rather than species. The method works with formalin fixed paraffin-embedded (FFPE) tissue and is compatible with the standard research IHC workflow in your lab.
Opal provides researchers with the tools to interrogate multiple pathways while retaining context provided by tissue images. This approach provides information not available from alternative techniques like flow cytometry or analysis of single markers in serial sections.
Opal will enable you to:
|Detection Method||MSI, Fluorescence|
|Fluorescent Agent Type||Opal Polaris 480, Opal 520, Opal 570, Opal 620, Opal 690, and Opal Polaris 780|
|Product Brand Name||Opal|
|Quantity in a Package Amount||1.0 per pack|
|Sample Type||Human FFPE|
|Shipping Condition||Blue Ice|
|Species Reactivity||Rabbit and Mouse Primary Antibodies|
|Unit Size||50 slides|
The Mantra™ quantitative pathology workstation, with inForm® image analysis software, enables easy visualization, quantification, and phenotyping of multiple types of immune cells simultaneously in intact FFPE tissue sections for cancer immunology research.