The Opal 4-Color Anti-Rabbit Automation IHC Kit is an immunohistochemistry detection kit that enables multiplex imaging of mouse tissue whilst retaining spatial context in an automated set up. It has been optimized for use on the Leica Biosystems BOND RX with mouse FFPE tissue and configured and validated for imaging on the Vectra®, Vectra Polaris™ and Mantra™ systems.
For research use only. Not for use in diagnostic procedures
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The Opal 4-Color Anti-Rabbit Automation IHC Kit contains:
Users should source primary antibodies and other workflow reagents separately. The Opal 4-Color Anti-Rabbit Automation IHC Kit has been optimized for use on the Leica Biosystems BOND RX with mouse FFPE tissue and configured and validated for imaging on the Vectra®, Vectra® Polaris™ and Mantra™ systems. These immunohistochemistry kits contain enough reagents to stain 50-slides at the recommended 1:150 dilution ratio.
What is Opal?
Opal multiplex IHC kits make multiplex methods accessible to researchers who work with standard immunohistochemistry (IHC). The Opal method allows use of any number of unlabeled primary antibodies from the same species in multiplexed tissue assays, with no fear of cross-reactivity. Antibodies for simultaneous IHC may now be selected based on performance, rather than species. The Opal IHC multiplexing method works with formalin fixed paraffin-embedded (FFPE) tissue and is compatible with the standard research IHC workflow in your lab.
Opal provides researchers with the tools to interrogate multiple pathways while retaining context provided by tissue images. This approach provides information not available from alternative techniques like flow cytometry or analysis of single markers in serial sections.
Opal will enable you to:
Automating Opal staining brings the benefit of:
|Detection Method||MSI, Fluorescence|
|Fluorophore||Opal 520, Opal 570 and Opal 689|
|Product Brand Name||Opal|
|Quantity in a Package Amount||1.0 per pack|
|Sample Type||Mouse or Human FFPE|
|Shipping Condition||Blue Ice|
|Unit Size||50 slides|