The NEXTFLEX® Small RNA-Seq Kit v3 with UDIs uses patented and patent-pending technology to provide a reduced-bias small RNA library preparation solution with Unique Dual Indexes (UDIs) for Illumina® sequencing. PerkinElmer's approach to reducing ligation-associated bias involves the use of adapters with randomized bases at the ligation junctions, resulting in greatly decreased bias in comparison to standard protocols.
For research use only. Not for use in diagnostic procedures.
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The NEXTFLEX small RNA-seq v3 kit with UDIs uses the same popular chemistry of the NEXTFLEX small RNA-seq kit v3, which incorporates technologies that reduce small RNA ligation bias, increase ligation efficiency, reduce adapter-dimer formation, and build in a new dimension of data quality, integrity, and accuracy with the integration of Unique Dual Indexes (UDIs) for Illumina® sequencing on the MiSeq®, HiSeq® 2000/2500, and NovaSeq® platforms. The addition of UDI barcodes for small RNA sequencing allows confident multiplexing of up to 384 samples, all while mitigating the risk of index hopping and spread of signal that can occur on a patterned flow cell. The purity of each index sequence is validated by sequencing for high confidence in the resulting sequencing data quality.
Learn more about small RNA-sequencing library prep.