Stable, recombinant ValiScreen® cell line expressing the Neuropeptide FF receptor 1 (NPFF1) receptor, human recombinant in CHO-K1 host cells.
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Cell Line Terms and Conditions must be accepted before orders are placed. Additional agreement needed for commercial use.
PerkinElmer’s ValiScreen® cell lines are provided in two vials of frozen cells, each containing ∽2.5 x 106 cells. Detailed product information as well as recommended cell culture conditions and validation assay date are available for each cell line.
Clonal selection for PerkinElmer’s ValiScreen cell lines is carried out with receptor binding. High and low expressors are then tested and selected for optimal binding and functional response according to the receptor specific transduction pathway.
PerkinElmer’s ValiScreen cell lines require accepted cell line Terms and Conditions prior to order processing. Additional agreement needed for commercial use. Contact us using the Request More Information button above for additional information regarding required documentation.
|Assay Validated||Binding, GTPγS, cAMP|
|Second Messenger Release||GTP exchange|
|Shipping Condition||Dry Ice|
|Special Ordering Information||Requires accepted Terms and Conditions. Additional agreement needed for commercial use.|
|Unit Size||5 million cells|
Aequorin is a photo protein originating from the jellyfish Aequorea Victoria. The apo-enzyme (apoaequorin) is a 21 kD protein, which requires a hydrophobic prosthetic group, coelenterazine, to be converted to aequorin,the active form of the enzyme. This enzyme possesses 3 calcium binding sites which control its activity.
Aequorin-based Ca2+ assays represent a new paradigm in drug discovery research for cell-based assays for Ca2+-coupled GPCRs and ion channels. In the aequorin assay, cells co-expressing apoaequorin and the target receptor are first incubated with the co-factor coelenterazinein order to reconstitute the activea equorin enzyme.
PerkinElmer’s validated, ready-to-use AequoZen™or cAMPZen™ frozen, irradiated cells make it easier for you to perform functional testing of GPCRs. Just thaw and use! Reliable, convenient AequoZen cells for aequorin calcium testing or cAMPZen cells for cAMP testing let you get the testing done. So you can concentrate on results.
Aequorin and Photina cell lines – the alternative calcium flux assay. PDL-coated microplates improve your performance