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neolite is a homogeneous, high sensitivity, long-lived glowtype firefly luciferase reporter gene assay system for the quantification of firefly luciferase expression in mammalian cells. The reagent formulation contains compounds that facilitate both cell lysis as well as the luciferase enzymatic reaction. neolite has been designed to cover the applications where a high light intensity is required together with an extended half-life. neolite is designed for systems that require increased sensitivity with the benefit of an extended detection time. neolite generates a luminescent signal that is both bright and stable. In general neolite has a signal half-life of more than 2 hours at high signal intensity. Large quantity bulk order quote available upon request. Please inquire with your local PerkinElmer representative.
|Experimental Type||In vitro|
|Product Brand Name||neolite|
|Quantity in a Package Amount||10000.0 Units|
|Unit Size||1,000 mL|
Over these last few decades there has been a growing trend in drug discovery to use cellular systems and functional assays, in addition to biochemical assays, for the characterization of new potential therapeutics. The ability to study the interaction between a candidate drug and its target within the context of a whole, intact cell allows for more physiologically relevant data to be obtained. However, such assays are more complex than traditional biochemical assays as such facts as membrane permeability, cellular metabolism, cell variability, additional binding partners, and signal transduction must be considered.
To help you navigate the complexities in designing cell-based assays, we have gathered insights collected over the years and compiled them to provide you with elements to consider when setting up your cell-based assays. After all, any assay, biochemical or cell-based, is only as good as its design.