The AlphaLISA® SureFire® Ultra™ Human and Mouse Phospho-SLP-76 (Ser376)/Total SLP-76 assay kit is used to measure both the phosphorylation of SLP-76 and total levels of endogenous SLP-76 in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure SLP-76 phosphorylation by either recombinant or endogenous receptors and can be applied to primary cells.
For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
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Lymphocyte cytosolic protein 2 (SLP-76 or LCP2) is a signal-transducing adaptor protein. ZAP-70 phosphorylates SLP-76, activating SLP-76 which then translocates to the plasma membrane and promotes a multi-protein complex, which participates in the activation, survival, and proliferation of T-Lymphocytes. SLP-76 is involved in multiple sclerosis, rheumatoid arthritis, psoriasis, and many types of cancers.
The AlphaLISA SureFire Ultra Multiplex Human and Mouse Phospho-SLP-76 (Ser376)/Total SLP-76 Detection Kit is used to measure both phosphorylated and total levels of endogenous SLP-76 in cellular lysates in a multiplexing Alpha no-wash assay. No specially engineered cell lines are required. The 615 nm (Eu) signal corresponds to the phosphorylated SLP-76 analysis, and the 545 nm (Tb) signal corresponds to the total SLP-76 analysis. This kit has been formulated to provide superior signal:background assay windows and to perform without interference in the presence of extraneous antibodies, making it amenable to the study of therapeutic and blocking antibodies.
In this assay, the Alpha 615 Acceptor bead is coated with the CaptSure™ antibody, which binds the CaptSure-tagged anti-phospho target antibody. The Alpha 545 Acceptor bead is coated with the CaptSure2™ agent, which binds the CaptSure2 tagged anti-total target protein antibody. The Alpha Donor bead binds the biotinylated anti-total target protein antibody.
|Assay Points (200-50,000)||500 Assay Points|
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA SureFire Ultra Multiplex|
|Shipping Condition||Blue Ice|
|Target Species||Human, Mouse|
|Therapeutic Area||Autoimmunity, Oncology, Immuno-oncology|
|Unit Size||500 Assay Points|
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