PerkinElmer
check quantity

AlphaLISA SureFire Ultra Multiplex Human and Mouse Phospho-SLP-76 (Ser376)/Total SLP-76 Detection Kit, 500 Assay Points

The AlphaLISA® SureFire® Ultra Human and Mouse Phospho-SLP-76 (Ser376)/Total SLP-76 assay kit is used to measure both the phosphorylation of SLP-76 and total levels of endogenous SLP-76 in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure SLP-76 phosphorylation by either recombinant or endogenous receptors and can be applied to primary cells.

For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

Part Number
Unit Size
List Price
Your Price
Quantity
MPSU-PTSLP-K500
500 Assay Points
3987.00 USD
 
more
MPSU-PTSLP-K10K
10,000 Assay Points
24100.00 USD
 
more
MPSU-PTSLP-K50K
50,000 Assay Points
75700.00 USD
 
more
MPSU-PTSLP-K-HV
100 Assay Points
1179.00 USD
 
more
Buy Now

Please enter valid quantity

Please log in to add favorites.

NULL OR EMPTY CART

Overview

Lymphocyte cytosolic protein 2 (SLP-76 or LCP2) is a signal-transducing adaptor protein. ZAP-70 phosphorylates SLP-76, activating SLP-76 which then translocates to the plasma membrane and promotes a multi-protein complex, which participates in the activation, survival, and proliferation of T-Lymphocytes. SLP-76 is involved in multiple sclerosis, rheumatoid arthritis, psoriasis, and many types of cancers.

The AlphaLISA SureFire Ultra Multiplex Human and Mouse Phospho-SLP-76 (Ser376)/Total SLP-76 Detection Kit is used to measure both phosphorylated and total levels of endogenous SLP-76 in cellular lysates in a multiplexing Alpha no-wash assay. No specially engineered cell lines are required. The 615 nm (Eu) signal corresponds to the phosphorylated SLP-76 analysis, and the 545 nm (Tb) signal corresponds to the total SLP-76 analysis. This kit has been formulated to provide superior signal:background assay windows and to perform without interference in the presence of extraneous antibodies, making it amenable to the study of therapeutic and blocking antibodies.

  • No-wash steps, no separation steps
  • Multiplexing assay
  • Sensitive detection
  • No interference from exogenous antibodies
  • Results in less than 3 hours

In this assay, the Alpha 615 Acceptor bead is coated with the CaptSure antibody, which binds the CaptSure-tagged anti-phospho target antibody. The Alpha 545 Acceptor bead is coated with the CaptSure2™ agent, which binds the CaptSure2 tagged anti-total target protein antibody. The Alpha Donor bead binds the biotinylated anti-total target protein antibody.

Specifications

Assay Points (200-50,000) 500 Assay Points
Assay Target SLP-76
Assay Technology Alpha
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA SureFire Ultra Multiplex
Shipping Condition Blue Ice
Target Species Human, Mouse
Therapeutic Area Autoimmunity, Oncology, Immuno-oncology
Unit Size 500 Assay Points
Resources, Events & More
  • All

Application Brief

Eight Limitations of ELISA and How to Overcome Them Using Alternative Technologies

The introduction of enzyme-linked immunosorbent assays (ELISAs) in the early 1970’s offered researchers a non-radiometric immunoassay platform without compromising sensitivity. Over the last 50 years scientists have made huge strides in disease research and drug discovery and a demand for greater ...

PDF 1 MB

Brochure

Alpha Technology Solutions

Product brochure for the Alpha Technology, a versatile, no wash, homogeneous assay technology that's suitable for a broad range of applications.

PDF 2 MB
Drug Discovery Screening Solutions Brochure

Find out about our range of integrated solutions for drug discovery screening in this e-brochure.

Our screening solutions for high-throughput screening, phenotypic screening and data analysis help to streamline drug discovery workflows in labs across the globe. Our portfolio includes automat ...

PDF 4 MB