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AlphaLISA SureFire Ultra Multiplex Human and Mouse Phospho-RET (panTyr)/Total RET Detection Kit, 10,000 Assay Points

The AlphaLISA® SureFire® Ultra Human and Mouse Phospho-RET (panTyr)/Total RET assay kit is used to measure both the phosphorylation of RET and total levels of endogenous RET in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure RET phosphorylation by either recombinant or endogenous receptors and can be applied to primary cells.

For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

Part Number
Unit Size
List Price
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MPSU-PTRET-K500
500 Assay Points
3987.00 USD
 
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MPSU-PTRET-K10K
10,000 Assay Points
24100.00 USD
 
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MPSU-PTRET-K50K
50,000 Assay Points
75700.00 USD
 
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MPSU-PTRET-K-HV
100 Assay Points
1179.00 USD
 
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Overview

Rearranged during transfection (RET or c-RET) is a receptor tyrosine kinase primarily expressed as two isoforms of 1072 & 1114 amino acids. RET activation by glial cell line-derived neurotrophic family ligands (GFL) leads to its dimerization and autophosphorylation on intracellular tyrosine residues. RET is required for the development of the nervous system and several other tissues. RET alterations have been detected in numerous human cancers. The development of highly selective and potent RET kinase inhibitors is therefore of great interest to treat RET-altered cancers.

The AlphaLISA SureFire Ultra Multiplex Human and Mouse Phospho-RET (panTyr)/Total RET Detection Kit is used to measure both phosphorylated and total levels of endogenous RET in cellular lysates in a multiplexing Alpha no-wash assay. No specially engineered cell lines are required. The 615 nm (Eu) signal corresponds to the phosphorylated RET analysis, and the 545 nm (Tb) signal corresponds to the total RET analysis. This kit has been formulated to provide superior signal:background assay windows and to perform without interference in the presence of extraneous antibodies, making it amenable to the study of therapeutic and blocking antibodies.

  • No-wash steps, no separation steps
  • Multiplexing assay
  • Sensitive detection
  • No interference from exogenous antibodies
  • Results in less than 3 hours

In this assay, the Alpha 615 Acceptor bead is coated with the CaptSure antibody, which binds the CaptSure-tagged anti-phospho target antibody. The Alpha 545 Acceptor bead is coated with the CaptSure2™ agent, which binds the CaptSure2 tagged anti-total target protein antibody. The Alpha Donor bead binds the biotinylated anti-total target protein antibody.

Specifications

Assay Points (200-50,000) 10000 Assay Points
Assay Target RET
Assay Technology Alpha
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA SureFire Ultra Multiplex
Shipping Condition Blue Ice
Target Species Human, Mouse
Therapeutic Area Oncology
Unit Size 10,000 Assay Points
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Brochure

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