The AlphaLISA® SureFire® Ultra™ Human Phospho-ALK (Tyr1604)/Total ALK assay kit is used to measure both the phosphorylation of ALK and total levels of endogenous ALK in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure ALK phosphorylation by either recombinant or endogenous receptors and can be applied to primary cells.
For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
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Anaplastic lymphoma kinase (ALK) also known as ALK tyrosine kinase receptor or CD246 (cluster of differentiation 246) is an enzyme that in humans is encoded by the ALK gene. ALK plays a pivotal role in cellular communication and in the normal development and function of the nervous system. The ALK gene can be oncogenic by forming a fusion gene with any of several other genes, by gaining additional gene copies or with mutations of the actual DNA code for the gene itself.
The AlphaLISA SureFire Ultra Multiplex Human Phospho-ALK (Tyr1604)/Total ALK Detection Kit is used to measure both phosphorylated and total levels of endogenous ALK in cellular lysates in a multiplexing Alpha no-wash assay. No specially engineered cell lines are required. The 615 nm (Eu) signal corresponds to the phosphorylated ALK analysis, and the 545 nm (Tb) signal corresponds to the total ALK analysis. This kit has been formulated to provide superior signal:background assay windows and to perform without interference in the presence of extraneous antibodies, making it amenable to the study of therapeutic and blocking antibodies.
In this assay, the Alpha 615 Acceptor bead is coated with the CaptSure™ antibody, which binds the CaptSure-tagged anti-phospho target antibody. The Alpha 545 Acceptor bead is coated with the CaptSure2™ agent, which binds the CaptSure2 tagged anti-total target protein antibody. The Alpha Donor bead binds the biotinylated anti-total target protein antibody.
|Assay Points (200-50,000)||100 Assay Points|
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA SureFire Ultra Multiplex|
|Shipping Condition||Blue Ice|
|Unit Size||100 Assay Points|
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