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AlphaLISA SureFire Ultra Human and Mouse Phospho-AKT 1/2/3 (Thr308)/Total AKT1 Detection Kit, 50,000 Assay Points

The AlphaLISA® SureFire® Ultra Human and Mouse Phospho-AKT 1/2/3 (Thr308)/Total AKT1 assay kit is used to measure both the phosphorylation of AKT 1/2/3 and total levels of endogenous AKT 1/2/3 in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure AKT 1/2/3 phosphorylation by either recombinant or endogenous receptors and can be applied to primary cells.

For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

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Unit Size
List Price
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MPSU-PTAKT-O500
500 Assay Points
3362.00 USD
 
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MPSU-PTAKT-O10K
10,000 Assay Points
20300.00 USD
 
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MPSU-PTAKT-O50K
50,000 Assay Points
63800.00 USD
 
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MPSU-PTAKT-O-HV
100 Assay Points
996.00 USD
 
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Overview

Protein kinase B (PKB) also known as AKT1 AKT2 and AKT3 encode the RAC alpha, beta, and gamma serine/threonine protein kinases respectively. AKT1 is involved in cellular survival pathways, by inhibiting apoptotic process. It is also able to induce protein synthesis pathways and is therefore a key signaling protein in the cellular pathways that lead to skeletal muscle hypertrophy and general tissue growth. Akt2 is an important signaling molecule in the insulin signaling pathway. AKT3 appears to be predominantly expressed in the brain.

The AlphaLISA SureFire Ultra Multiplex Human and Mouse Phospho-AKT 1/2/3 (Thr308)/Total AKT1 Detection Kit is used to measure phosphorylated AKT 1/2/3 and total levels of AKT1 in cellular lysates in a multiplexing Alpha no-wash assay. No specially engineered cell lines are required. The 615 nm (Eu) signal corresponds to the phosphorylated AKT 1/2/3, and the 545 nm (Tb) signal corresponds to the total AKT1 analysis. This kit has been formulated to provide superior signal:background assay windows and to perform without interference in the presence of extraneous antibodies, making it amenable to the study of therapeutic and blocking antibodies.

  • No-wash steps, no separation steps
  • Multiplexing assay
  • Sensitive detection
  • No interference from exogenous antibodies
  • Results in less than 3 hours

In this assay, the Alpha 615 Acceptor bead is coated with the CaptSure antibody, which binds the CaptSure-tagged anti-phospho target antibody. The Alpha 545 Acceptor bead is coated with the CaptSure2™ agent, which binds the CaptSure2 tagged anti-total target protein antibody. The Alpha Donor bead binds the biotinylated anti-total target protein antibody.

Specifications

Assay Points (200-50,000) 50000 Assay Points
Assay Target AKT 1/2/3
Assay Technology Alpha
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA SureFire Ultra Multiplex
Shipping Condition Blue Ice
Target Species Human, Mouse
Therapeutic Area Oncology
Unit Size 50,000 Assay Points
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Application Brief

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Brochure

Alpha Technology Solutions

Product brochure for the Alpha Technology, a versatile, no wash, homogeneous assay technology that's suitable for a broad range of applications.

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Drug Discovery Screening Solutions Brochure

Find out about our range of integrated solutions for drug discovery screening in this e-brochure.

Our screening solutions for high-throughput screening, phenotypic screening and data analysis help to streamline drug discovery workflows in labs across the globe. Our portfolio includes automated liquid handling, assays and reagents, imaging and detection systems, and informatics.

Working independently or together, with our solutions you can achieve consistent and accurate results. By accelerating the identification and characterization of effective and safe drug candidates, the PerkinElmer portfolio enables you to optimize efficiency in your lab and deliver more actionable, real-world results.

Download the brochure to learn more about how we can partner with you so that you can discover smarter, more effective, data-driven breakthroughs in the critical screening stages of drug discovery and development.

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