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Membrane Target Systems are quality assured frozen membranes from cells that express recombinant or endogenous receptors. We submit every batch of receptor to stringent quality control testing that includes saturation radioligand binding assay to determine receptor concentration (Bmax) and affinity (Kd). Competition binding assays are performed determine affinity (Ki) against known reference agonists and antagonists. GTPgS data is also provided for some of our Gi coupled receptors.
Membranes are carefully prepared and ready for a variety of HTS applications, including radioligand binding (using either proximity methods, such as FlashPlate, or classical filtration methods).
Products are packaged as frozen crude membrane preparations. One assay unit is defined as micrograms of protein, defined by competition binding assay (filtration). A complete product description and recommended protocol are included on the Product Information Sheet (see Certificate of Analysis).
|Assay Target Class||GPCR|
|Assay Target Type||Membrane preparation|
|Product Brand Name||Membrane Target Systems|
|Shipping Condition||Dry Ice|
|Unit Size||400 units|