PerkinElmer
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MaxSignal® HTS Chloramphenicol ELISA Kit

Designed and developed specifically for the aquaculture industry, the MaxSignal® HTS Nitrofurans and Chloramphenicol ELISA Assays and DS2 Automated ELISA Platform solution delivers a simple, simultaneous 5-in-1 sample preparation method for AOZ, AMOZ, SEM and AHD Nitrofurans as well as Chloramphenicol. This saves time, hands-on requirements, reagents and lab bench footprint and helps support faster and more informed decision making around daily, incoming seafood lots. Cross-contamination will also be reduced and the solution provides excellent sample traceability when using the integrated bar-code scanner and can be linked to LIMS for easy result recording and sharing.

Part Number
Type
FOOD-4001-01
Chloramphenicol
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FOOD-4002-01
Furazolidone (AOZ)
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FOOD-4003-01
Furaltadone (AMOZ)
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FOOD-4004-01
Nitrofurazone (SEM)
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FOOD-4005-01
Nitrofurantoin (AHD)
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Overview

The MaxSignal® Chloramphenicol ELISA Kit is a competitive ELISA for the quantitative analysis of chloramphenicol in a variety of sample types. Chloramphenicol is a broad spectrum antibiotic which is frequently employed in animal production for its excellent antibacterial and pharmacokinetic properties. However, in humans it leads to hematotoxic side effects. Historically, chloramphenicol residues have been determined either radioimmunologically or gas chromatographically. However, enzyme immunoassays (ELISA) show considerable advantages over conventional methods. Radioactive materials are no longer required, and the processing time is considerably less than the gas chromatographic method.

MaxSignal® HTS Chloramphenicol ELISA Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the entire kit at 2–8°C. Do not use this product past the expiration date indicated on the Certificate of Analysis.

SENSITIVITY

  • Detection Limit (ng/g or ppb) of Shrimp is 0.1

SPECIFICITY

  • Analyte Cross-Reactivity (%)
  • Chloramphenicol 100.0
  • AOZ<1.0
  • AMOZ<1.0
  • AHD<1.0
  • SEM<1.0
  • Thiamphenicol:0.4
  • Tetracyclines<0.01
  • Gentamicin<0.01
  • Ampicillin<0.01
  • Florfenicol<0.01

Specifications

Type Chloramphenicol
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Application Note

High Throughput Screening Solutions for Nitrofurans and Chloramphenicol in Shrimp Samples

The use of nitrofurans, a class of broad-spectrum antibiotics widely used in the aquaculture industry and their metabolites, has been regulated by several countries and organizations across the world for their harmful effects on human health. ELISA assays are widely used for the detection of nitrofuran metabolites and chloramphenicol for regulatory conformance owing to the high sensitivity, selectivity and ease of use of the method. In this study, we demonstrate the accuracy and precision (CCß validation study) of the simultaneous 5-in-1 sample extraction method by manual ELISA and DS2 automation methods. Sensitivity of the assay kits (LOD) was also demonstrated using manual and DS2 method. Finally, sample variability testing was performed to characterize the effects of matrix from various shrimp sources.

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Brochure

MaxSignal HTS Automated Seafood Solution Brochure

This brochure provides information about the MaxSignal HTS automated seafood solution.

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FAQ

MaxSignal HTS Automated Seafood Solution FAQ

This FAQ provides information about the MaxSignal HTS 5-in-1 ELISA and DS2 Automated Laboratory ELISA platform.

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Flyer

MaxSignal HTS Automated Seafood Solution Sell Sheet

This sell sheet provides information about the MaxSignal HTS 5-in-1 ELISA and DS2 Automated Laboratory ELISA platform.

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Specification Sheet

MaxSignal HTS Automated Seafood Solution Specification Sheet

This specification sheet provides information about the MaxSignal HTS 5-in-1 ELISA and DS2 Automated Laboratory ELISA platform.

PDF 380 KB