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TREM2 LANCE Ultra TR-FRET Detection Kit, 10,000 assay points

The LANCE Ultra TREM2 detection kit is designed for the detection of TREM2 in cell supernatants using a simple, homogeneous LANCE Ultra assay (no wash steps).

For research use only. Not for use in diagnostic procedures.

Part Number
Unit Size
List Price
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TRF4030C
500 Assay Points
1512.00 USD
 
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TRF4030M
10,000 Assay Points
6800.00 USD
 
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Overview

Please note control supernatant is sold separately, catalog number TRF4030S.

Formats:

  • Our 500 assay point kit allows you to run 500 wells in 384-well format, using a 20 µL reaction volume.
  • Our 10,000 assay point kit allows you to run 10,000 wells in 384-well format, using a 20 µL reaction volume.

LANCE® and LANCE® (Lanthanide chelate excite) Ultra are homogeneous (no wash) TR-FRET (time-resolved fluorescence resonance energy transfer) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second antibody is labeled with an acceptor fluorophore [ULight™ dye]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm. Data are represented as ratiometric (665/615 nm X 10,000).

TREM2 is a transmembrane molecule expressed on myeloid cells. It acts as the receptor for an unknown ligand to activate myeloid cells such as dendritic cells, increasing phagocytic activity. Recently, TREM2 has been shown to be involved in neurodegenerative diseases such as ataxia, early dementia and Alzeimer’s disease. Elevated levels of TREM2 have been noted in cerebrospinal fluid as a response to Alzeimer’s disease.

Specifications

Assay Target TREM2
Assay Target Class Protein
Automation Compatible Yes
Detection Method Time-Resolved Fluorescence (TRF), TR-FRET
Product Brand Name LANCE Ultra
Shipping Condition Blue Ice
Unit Size 10,000 Assay Points
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Manual

Protocol for LANCE Ultra cellular TREM2 assay kit

This manual describes how to run the LANCE Ultra cellular TREM2 assay kit.

PDF 476 KB