PerkinElmer
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Total STAT1 LANCE Ultra TR-FRET Detection Kit, 10,000 assay points

LANCE Ultra total STAT1 kits are designed for the detection of STAT1 (both phosphorylated and non-phosphorylated) in cell lysates using a simple, homogeneous LANCE Ultra TR-FRET assay (no wash steps). This assay is compatible with both adherent and suspension cells, and is intended to be used as a control or normalization assay in conjunction with the LANCE phospho-STAT1 assay.

For research use only. Not for use in diagnostic procedures.

Part Number
Unit Size
List Price
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TRF4029C
500 Assay Points
1512.00 USD
 
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TRF4029M
10,000 Assay Points
6800.00 USD
 
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Overview

Please note control lysates are sold separately, catalog number TRF4028S.

Formats:

  • Our 500 assay point kit allows you to run 500 wells in 384-well format, using a 20 µL reaction volume.
  • Our 10,000 assay point kit allows you to run 10,000 wells in 384-well format, using a 20 µL reaction volume.

LANCE® and LANCE® (Lanthanide chelate excite) Ultra are homogeneous (no wash) TR-FRET (time-resolved fluorescence resonance energy transfer) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second antibody is labeled with an acceptor fluorophore [ULight™ dye]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm. Data are represented as ratiometric (665/615 nm X 10,000).

STAT1 is an important transcriptional activator, and is involved in regulating cytokines and growth factor receptors. STAT1 is phosphorylated at Tyr-701 by receptor associated kinases, upon which STAT1 will dimerize (sometimes forming a heterodimer with STAT3), translocate to the nucleus, and work as transcription factors. Mutations of STAT1 result in either gain of function (resulting in CMC) or loss of function (causing impaired response to infection).

Specifications

Assay Pathway JAK/STAT
Assay Target STAT1
Assay Target Class Protein
Automation Compatible Yes
Detection Method Time-Resolved Fluorescence (TRF), TR-FRET
Product Brand Name LANCE Ultra
Shipping Condition Blue Ice
Unit Size 10,000 Assay Points
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Manual

Protocol for LANCE Ultra cellular total STAT1 assay kit

This manual describes how to run the LANCE Ultra cellular total STAT1 assay kit.

PDF 492 KB