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The LANCE® Ultra Human PTEN Detection Kit is designed for detection and quantitation of human PTEN in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.
Phosphatase and tensin homolog (PTEN) is a 402 AA protein that is expressed ubiquitously in the body. The main function of PTEN is to dephosphorylate phosphatidylinositol 3,4,5-triphosphate whereby resulting in the inhibition of the AKT signaling pathway; a pathway necessary for cell survival. Through this interaction PTEN was determined to play a crucial role in tumor suppression. In cancers like prostate, endometrial, lung and breast, PTEN expression is commonly lost or highly reduced. From the established connections between PTEN and cancer, PTEN is a highly sought after cancer biomarker.
|Assay Target Class||Protein|
|Detection Method||Time-Resolved Fluorescence (TRF), TR-FRET|
|Experimental Type||In vitro|
|Product Brand Name||LANCE Ultra|
|Shipping Condition||Blue Ice|
|Unit Size||10,000 Assay Points|
The introduction of enzyme-linked immunosorbent assays (ELISAs) in the early 1970’s offered researchers a non-radiometric immunoassay platform without compromising sensitivity. Over the last 50 years scientists have made huge strides in disease research and drug discovery and a demand for greater assay throughput and sensitivity has evolved. In response, more robust immunoassays have been developed to address some of the limitations of the standard, colorimetric ELISA.
Find out about the most common limitations of traditional ELISAs and how different ELISA alternative technologies address these limitations.