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The LANCE Ultra Residual Protein A Detection Kit is designed for detection and quantitation of Residual Protein A in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
The 500 point kit contains enough reagents to run 500 wells in 384-well format, using a 20 µL reaction volume (15 µL of sample). The 10,000 point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 µL reaction volume (15 µL of sample).
LANCE and LANCE (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.
Protein A is a 42 kDa protein widely used in affinity chromatography to purify antibodies, most importantly antibody therapeutics. The native form of Protein A from S. Aureus, in addition to many modified recombinant Protein A variants, are used in chromatography. The strong affinity and the high selectivity of Protein A for antibodies lead to an effective removal of host cell proteins. However, Protein A can be leached from columns to contaminate the antibody preparation. Since Protein A could elicit mitogenic or immunological reactions, there are strict regulations as to the acceptable levels of Protein A in antibody preparations. Therefore, there is a current need to quantitate Protein A in antibody preparations with excellent sensitivity and robustness, while maintaining high throughput capabilities.
|Assay Target||Protein A|
|Assay Target Class||Protein|
|Experimental Type||In vitro|
|Product Brand Name||LANCE Ultra|
|Shipping Condition||Blue Ice|
|Unit Size||500 Assay Points|