Residual Protein A LANCE Ultra TR-FRET Detection Kit, 10,000 assay points

The LANCE Ultra Residual Protein A Detection Kit is designed for detection and quantitation of Residual Protein A in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

The 500 point kit contains enough reagents to run 500 wells in 384-well format, using a 20 µL reaction volume (15 µL of sample). The 10,000 point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 µL reaction volume (15 µL of sample).

• No-wash steps, no separation steps
• TR-FRET technology
• Sensitive detection
• High reproducibility
• Faster time-to-results
• Easy automation
• 96-well, 384-well, and 1536-well formats

LANCE and LANCE (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight^™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.

Protein A is a 42 kDa protein widely used in affinity chromatography to purify antibodies, most importantly antibody therapeutics. The native form of Protein A from S. Aureus, in addition to many modified recombinant Protein A variants, are used in chromatography. The strong affinity and the high selectivity of Protein A for antibodies lead to an effective removal of host cell proteins. However, Protein A can be leached from columns to contaminate the antibody preparation. Since Protein A could elicit mitogenic or immunological reactions, there are strict regulations as to the acceptable levels of Protein A in antibody preparations. Therefore, there is a current need to quantitate Protein A in antibody preparations with excellent sensitivity and robustness, while maintaining high throughput capabilities.