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p53 (human) LANCE Ultra TR-FRET Detection Kit, 500 assay points

The LANCE® Ultra p53 Detection Kit is designed for detection and quantitation of human p53 in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

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500 Assay Points
2541.00 USD
10,000 Assay Points
11700.00 USD
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The LANCE® Ultra Human p53 Detection Kit is designed for detection and quantitation of p53 in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

  • No-wash steps, no separation steps
  • TR-FRET technology
  • Sensitive detection
  • High reproducibility
  • Faster time-to-results
  • Easy automation
  • 96-well, 384-well, and 1536-well formats

LANCE and LANCE (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore [ULight dye]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.

Tumor protein p53, also known as p53, is a well conserved phosphoprotein composed of 390 amino acids. The p53 protein is a multi-functional transcription factor that regulates cellular decisions regarding proliferation, cell cycle checkpoints, and apoptosis. The p53 protein plays a major role in cellular response to DNA damage and other genomic aberrations. The loss of the p53 supressor function through mutations or inactivation of its pathway is associated with most if not all human cancers. Activation of p53 can lead to cell cycle arrest, DNA repair or apoptosis. p53 is phosphorylated at multiple sites by several different protein kinases. This kit quantifies the total amount of p53 in human serum, cell culture supernatants, and cell lysates.


Assay Target p53
Assay Target Class Protein
Automation Compatible Yes
Detection Method Time-Resolved Fluorescence (TRF), TR-FRET
Product Brand Name LANCE Ultra
Shipping Condition Blue Ice
Therapeutic Area Cancer
Unit Size 500 Assay Points
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Manual: LANCE Ultra human p53 Detection Kit

This manual describes how to run a LANCE Ultra TR-FRET human p53 detection assay.

PDF 384 KB