For research use only. Not for use in diagnostic procedures.
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The LANCE® Ultra Human MMP-8 Detection Kit is designed for detection and quantitation of human matrix metalloproteinase 8 in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.
Human matrix metalloproteinase 8 (MMP8, Neutrophil collagenase or PMNL collagenase), a member of the matrix metalloproteinase family, is an enzyme composed of a pro-domain that is cleaved off for activation. MMP 8 is involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Increased serum levels of MMP8 have observed to be a strong predictor of mortality in patients with Rheumatoid Arthritis. Further, MMP8 serum levels show evidence of being a valuable biomarker for cancers such as colorectal cancer. This kit has been designed for the detection of Human MMP 8 in cell culture supernatants.
|Assay Target Class||Protein|
|Detection Method||Time-Resolved Fluorescence (TRF), TR-FRET|
|Experimental Type||In vitro|
|Product Brand Name||LANCE Ultra|
|Shipping Condition||Blue Ice|
|Unit Size||500 Assay Points|