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The LANCE Ultra Mouse IL-1β Detection Kit is designed for detection and quantitation of mouse interleukin 1β in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
The 500 point kit contains enough reagents to run 500 wells in 384-well format, using a 20 µL reaction volume (15 µL of sample). The 10,000 point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 µL reaction volume (15 µL of sample).
LANCE and LANCE (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.
The mouse Interleukin 1 beta (IL1ß) is produced as a 269 amino acid precursor that matures by proteolysis to its 152 amino acid active form. In humans, IL1α and IL1ß are central players of the immune response, displaying roles in inflammation both at local and systemic levels. IL1ß is functionally equivalent to IL1α. Its production has been reported in many cell types including brain cells, as well as monocytic and peripheral blood mononuclear cells. Among the biological activities of IL1 is the stimulation of T-helper cells, which then secrete IL2 and express IL2 receptor. IL1 acts directly on B-cells, promoting their proliferation and the synthesis of immunoglobulins. It supports tumor cytotoxicity mediated by monocytes and induces tumor regression. It has been shown that IL1 also promotes wound healing.
| Assay Target | IL1β |
|---|---|
| Assay Target Class | Cytokine |
| Automation Compatible | Yes |
| Experimental Type | In vitro |
| Product Brand Name | LANCE Ultra |
| Shipping Condition | Blue Ice |
| Therapeutic Area | Inflammation |
| Unit Size | 500 Assay Points |
The introduction of enzyme-linked immunosorbent assays (ELISAs) in the early 1970’s offered researchers a non-radiometric immunoassay platform without compromising sensitivity. Over the last 50 years scientists have made huge strides in disease research and drug discovery and a demand for greater assay throughput and sensitivity has evolved. In response, more robust immunoassays have been developed to address some of the limitations of the standard, colorimetric ELISA.
Find out about the most common limitations of traditional ELISAs and how different ELISA alternative technologies address these limitations.
