For research use only. Not for use in diagnostic procedures.
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The LANCE® Ultra Human MBNL1 Detection Kit is designed for detection and quantitation of human muscleblind-like protein 1 (MBNL1) in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.
Muscleblind-like proteins (MBNL) belong to a family of tissue-specific precursor and mature mRNA metabolism regulators in mammals. Muscleblind-like Protein 1 (MBNL1) regulates alternative splicing of pre-mRNA; it is found primarily in skeletal and cardiac muscle tissues. Increased expression of MBNL1 has been linked to myotonic dystrophy, a particular type of muscular dystrophy. The present kit permits the detection of human MBNL1 (i.e. analyte) in cell culture lysates and samples.
|Assay Target Class||Protein|
|Detection Method||Time-Resolved Fluorescence (TRF), TR-FRET|
|Product Brand Name||LANCE Ultra|
|Shipping Condition||Blue Ice|
|Unit Size||500 Assay Points|
This manual describes how to run a LANCE Ultra TR-FRET human MBNL1 detection assay.