For research use only. Not for use in diagnostic procedures.
The LANCE® Ultra™ Mouse/Rat IL-17A Detection Kit is designed for detection and quantitation of mouse or rat interleukin-17A in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
This is a replacement for TRF1514.
The LANCE® Ultra™ Mouse/Rat IL-17A Detection Kit is designed for detection and quantitation of mouse or rat interleukin-17A in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
The 500 point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume (15 μL of sample). The 10,000 point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume (15 μL of sample).
LANCE and LANCE (Lanthanide chelate excite) Ultra are TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (˜10 nm). This results in the emission of light at 665 nm.
Assay Target | IL-7A |
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Assay Target Class | Cytokine |
Assay Technology | LANCE Ultra |
Automation Compatible | Yes |
Detection Method | Time-Resolved Fluorescence (TRF), TR-FRET |
Experimental Type | In vitro |
Shipping Condition | Blue Ice |
Therapeutic Area | Inflammation |
Unit Size | 500 Assay Points |