IL-17A (mouse/rat) LANCE Ultra TR-FRET Detection Kit, 500 Assay Points

The LANCE® Ultra™ Mouse/Rat IL-17A Detection Kit is designed for detection and quantitation of mouse or rat interleukin-17A in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

This is a replacement for TRF1514.

The LANCE® Ultra™ Mouse/Rat IL-17A Detection Kit is designed for detection and quantitation of mouse or rat interleukin-17A in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

The 500 point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume (15 μL of sample). The 10,000 point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume (15 μL of sample).

• No-wash steps, no separation steps
• TR-FRET technology
• Sensitive detection
• High reproducibility
• Faster time-to-results
• Easy automation
• 96-well, 384-well, and 1536-well formats

LANCE and LANCE (Lanthanide chelate excite) Ultra are TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (˜10 nm). This results in the emission of light at 665 nm.