LC3B (human) LANCE Ultra TR-FRET Detection Kit, 500 Assay Points

The LANCE^® Ultra Human LC3B Detection Kit is designed for detection and quantitation of human LC3B in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

• No-wash steps, no separation steps
• TR-FRET technology
• Sensitive detection
• High reproducibility
• Faster time-to-results
• Easy automation
• 96-well, 384-well, and 1536-well formats

LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.

LC3 represents a mammalian homologue of the yeast autophagy related gene ATG8. It was originally characterized as light chain 3 of the microtubule associated protein 1 (MAP1LC3). The protein family consists of LC3 A, B, and C and the GABARAP subfamily. Human LC3B is 125 amino acids long. After synthesis, it is cleaved by ATG4B to expose a Cterminal glycine, representing the cytosolic form LC3B I. During autophagy the C-terminus is covalently linked to autophagosomal vesicle membranes via a phospholipid anchor and this form is called LC3B II. The transformation of LC3B I to II is mediated by a ubiquitination-like process involving ATG7 (E1), ATG3 (E2) and the ATG16L complex (E3). To date, LC3B is considered as the most persistent marker of the autophagy pathway.