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The LANCE® Ultra Human Kappa Light Chain Detection Kit is designed for detection and quantitation of human kappa light chain in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.
Most mammal IgG class antibodies, including those from human are composed of two heavy and two light chains. Such light chains can be of kappa or lambda types, with an expected ratio of 3 kappas to one lambda. Both appear to play the same function in antibodies. Free light chains are usually rare in blood. However, several types of multiple myelomas will secrete free light chains, which can then accumulate in organs such as the kidney and cause severe damage, with lambda chains being the poorest diagnostic. Knowledge of the type of light chain generated is also important for the creation and production of therapeutic antibodies. Techniques used for purification, such as protein L or anti species antibodies, can have different affinities for the two chains.
|Assay Target||Kappa light chain|
|Assay Target Class||Antibody|
|Detection Method||Time-Resolved Fluorescence (TRF), TR-FRET|
|Experimental Type||In vitro|
|Product Brand Name||LANCE Ultra|
|Shipping Condition||Blue Ice|
|Unit Size||500 Assay Points|
The introduction of enzyme-linked immunosorbent assays (ELISAs) in the early 1970’s offered researchers a non-radiometric immunoassay platform without compromising sensitivity. Over the last 50 years scientists have made huge strides in disease research and drug discovery and a demand for greater assay throughput and sensitivity has evolved. In response, more robust immunoassays have been developed to address some of the limitations of the standard, colorimetric ELISA.
Find out about the most common limitations of traditional ELISAs and how different ELISA alternative technologies address these limitations.
This manual explains how to run the LANCE Ultra human kappa light chain detection assay.