IL-12/p40 (human) LANCE Ultra TR-FRET Detection Kit, 500 Assay Points

The LANCE^® Ultra Human IL-12/p40 Detection Kit is designed for detection and quantitation of human IL-12 in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

• No-wash steps, no separation steps
• TR-FRET technology
• Sensitive detection
• High reproducibility
• Faster time-to-results
• Easy automation
• 96-well, 384-well, and 1536-well formats

LANCE^® and LANCE^® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight^™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.

Interleukin 12 p40 (IL12 p40) (also known as IL-12B, natural killer cell stimulatory factor 2, or cytotoxic lymphocyte maturation factor 2, p40) is a subunit of interleukin 12. It also serves as a subunit of Interleukin 23. This cytokine is involved in atopic diseases, asthma, eczema, rhinitis, and autoimmune diseases. IL12 appears to play a major role in auto-immune disease, in the resistance to bacterial and parasitic infections, in antiviral responses, and in the promotion of antitumor immunity. Increased plasma levels of IL12 p40 have been reported in certain neurological disorders and autoimmune diseases.