For research use only. Not for use in diagnostic procedures.
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The LANCE® Ultra Human IgM Detection Kit is designed for detection and quantitation of human IgM in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.
Immunoglobulin M (IgM) is the largest antibody produced by B cells. It is mainly produced in a pentameric form weighing around 900 kDa, but also in a hexameric form. Its multimeric nature gives IgM high avidity. It is mostly found in the serum, yet it is also present in secretions. IgM is a very efficient activator of the complement and appears very early during the infection process. Moreover, anti-A and anti-B antibodies responsible for blood type incompatibility are IgM molecules. Selective IgM deficiency (SIgMD) is a very rare immune disorder that results in absence or deficiency of IgM, with normal levels of other immunoglobulins, namely IgA and IgG. Hyper-IgM syndrome (HIGM) is characterized by a decrease or absence of IgG and IgA, whereas the IgM level is normal or increased.
|Assay Target Class||Antibody|
|Detection Method||Time-Resolved Fluorescence (TRF), TR-FRET|
|Experimental Type||In vitro|
|Product Brand Name||LANCE Ultra|
|Shipping Condition||Dry Ice|
|Unit Size||10,000 Assay Points|