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The LANCE® Ultra Human IgE Detection Kit is designed for detection and quantitation of human IgE in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.
Immunoglobulin E (IgE) is a monomeric antibody with four Ig-like domains. Compared to all other immunoglobulin classes, IgE is typically the least abundant isotype in plasma of normal healthy individuals. IgE binds with extremely high affinity to Fc-epsilon receptor I (FcɛRI), which is expressed on mast cells, basophils, Langerhans cells, and eosinophils. Circulating IgE upregulates FcɛRI expression on these cells. The combination of strong binding and upregulation of FcɛRI expression contributes to the remarkable potency of this immunoglobulin. IgE is associated with hypersensitivity and allergic reactions, as well as the response to parasitic worm infections.
|Assay Target Class||Antibody|
|Detection Method||Time-Resolved Fluorescence (TRF), TR-FRET|
|Experimental Type||In vitro|
|Product Brand Name||LANCE Ultra|
|Shipping Condition||Blue Ice|
|Unit Size||10,000 Assay Points|
The introduction of enzyme-linked immunosorbent assays (ELISAs) in the early 1970’s offered researchers a non-radiometric immunoassay platform without compromising sensitivity. Over the last 50 years scientists have made huge strides in disease research and drug discovery and a demand for greater assay throughput and sensitivity has evolved. In response, more robust immunoassays have been developed to address some of the limitations of the standard, colorimetric ELISA.
Find out about the most common limitations of traditional ELISAs and how different ELISA alternative technologies address these limitations.
This manual explains how to run the LANCE Ultra TR-FRET human IgE detection assay.