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The LANCE® Ultra Human Perlecan Detection Kit is designed for detection and quantitation of human perlecan in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.
Perlecan is a large (4391 AA) multidomain protein, one of them being Endorepellin (3687 – 4391 AA), synthesized primarily by vascular endothelial cells and deposited in the vascular extracellular matrix. Its function is to crosslink a variety of matrix components to maintain the endothelial barrier function. Further, it can stimulate growth and regeneration of the endothelium via the modulation of certain growth factor activities. Perlecan has garnered significant interest due to its ability to suppress tumor growth upon its own suppression. Also, its levels have been observed to decrease in other diseases like atherosclerosis and diabetes.
|Assay Target Class||Protein|
|Detection Method||Time-Resolved Fluorescence (TRF), TR-FRET|
|Experimental Type||In vitro|
|Product Brand Name||LANCE Ultra|
|Shipping Condition||Blue Ice|
|Unit Size||10,000 Assay Points|
The introduction of enzyme-linked immunosorbent assays (ELISAs) in the early 1970’s offered researchers a non-radiometric immunoassay platform without compromising sensitivity. Over the last 50 years scientists have made huge strides in disease research and drug discovery and a demand for greater assay throughput and sensitivity has evolved. In response, more robust immunoassays have been developed to address some of the limitations of the standard, colorimetric ELISA.
Find out about the most common limitations of traditional ELISAs and how different ELISA alternative technologies address these limitations.