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The LANCE Ultra Human CXCL9/MIG Detection Kit is designed for detection and quantitation of human CXCL9/MIG in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
The 500 point kit contains enough reagents to run 500 wells in 384-well format, using a 20 µL reaction volume (15 µL of sample). The 10,000 point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 µL reaction volume (15 µL of sample).
LANCE and LANCE (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.
C-X-C Motif Chemokine 9 (CXCL9), previously called MIG, is a 14 kDa protein belonging to the intercrine alpha (chemokine CXC) family. Its induction is enhanced by TNFα in dermal fibroblasts and vein endothelial cells. The synthesis of CXCL9 is specifically induced in macrophages, monocytes, neutrophils, APC, B cells, and eosinophils by IFNγ and mediated via the JAK-STAT signaling pathway. The main function of this chemokine is the recruitment of leukocytes to sites of infection and inflammation. Some studies have shown that CXCL9 is active against Gram-negative and Gram-positive bacteria. CXCL9 may play a role as a mediator of T-cell recruitment and activation in some diseases like psoriasis and pulmonary disease. CXCL9 is expressed in allogeneic skin grafts several days before completion of rejection.
| Assay Target | CXCL9 |
|---|---|
| Assay Target Class | Cytokine |
| Automation Compatible | Yes |
| Experimental Type | In vitro |
| Product Brand Name | LANCE Ultra |
| Shipping Condition | Blue Ice |
| Therapeutic Area | Inflammation |
| Unit Size | 500 Assay Points |
The introduction of enzyme-linked immunosorbent assays (ELISAs) in the early 1970’s offered researchers a non-radiometric immunoassay platform without compromising sensitivity. Over the last 50 years scientists have made huge strides in disease research and drug discovery and a demand for greater assay throughput and sensitivity has evolved. In response, more robust immunoassays have been developed to address some of the limitations of the standard, colorimetric ELISA.
Find out about the most common limitations of traditional ELISAs and how different ELISA alternative technologies address these limitations.
