For research use only. Not for use in diagnostic procedures.
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The LANCE Ultra Human IL-4 Detection Kit is designed for detection and quantitation of human interleukin 4 in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
The 500 point kit contains enough reagents to run 500 wells in 384-well format, using a 20 µL reaction volume (15 µL of sample). The 10,000 point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 µL reaction volume (15 µL of sample).
LANCE and LANCE (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.
Human Interleukin 4 (IL4), also known as B Cell Stimulatory Factor 1, is an anti-inflammatory glycoprotein of 129 amino acids. It is produced by a variety of cells including Th2, mast cells, and basophils. Via binding to type 1 IL4 receptor, IL4 acts on hematopoietic cells and promotes class switching to IgE. A high level of IL4 has been associated with an increased production of IgE and allergy. This cytokine suppresses IFN-γ and IL-8 production. It inhibits the production of inflammatory cytokines (IL1, IL6, and TNFα). In vivo, injection of IL4 has been shown to protect against experimental arthritis and immune complex-induced lung inflammation in rats. It could potentially be used in the treatment of chronic inflammatory diseases. IL4 may also play a role in the pathogenesis of chronic lymphocytic leukemia.
|Detection Method||Time-Resolved Fluorescence (TRF), TR-FRET|
|Experimental Type||In vitro|
|Product Brand Name||LANCE Ultra|
|Shipping Condition||Blue Ice|
|Unit Size||500 Assay Points|