IL-5 (human) LANCE Ultra TR-FRET Detection Kit, 10,000 assay points

The LANCE Ultra Human IL-5 Detection Kit is designed for detection and quantitation of human interleuukin-5 in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

The 500 point kit contains enough reagents to run 500 wells in 384-well format, using a 20 µL reaction volume (15 µL of sample). The 10,000 point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 µL reaction volume (15 µL of sample).

• No-wash steps, no separation steps
• TR-FRET technology
• Sensitive detection
• High reproducibility
• Faster time-to-results
• Easy automation
• 96-well, 384-well, and 1536-well formats

LANCE and LANCE (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight^™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.

Human Interleukin 5 (IL5) is produced as a precursor protein maturing into a 115 amino acid protein. The mature active protein is heavily glycosylated and is covalently linked in a homodimer by a disulfide bond, with a native apparent molecular weight of 45-60 kDa. It is produced by Th2 cells, mast cells, and eosinophils. IL5 stimulates B cell growth and promotes the production of cytotoxic T-cells from thymocytes, but its key function is to mediate activation, maturation, and survival of eosinophils. IL5 activated eosinophils eliminate antibody bound parasites through the release of cytotoxic granule proteins. IL5 seems to play a major role in the development of allergic disease/asthma response, which makes IL5 an interesting target for the development of anti-allergy drugs. IL5 is also a potential marker of acute graft-versus-host disease.