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The LANCE Ultra Human IL-1α Detection Kit is designed for detection and quantitation of human interleuukin-1α in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
The 500 point kit contains enough reagents to run 500 wells in 384-well format, using a 20 µL reaction volume (15 µL of sample). The 10,000 point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 µL reaction volume (15 µL of sample).
LANCE and LANCE (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.
Interleukin 1α (IL1α) is an 18 kDa pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. The IL1 family includes the agonists IL1α and IL1β and the antagonist IL1RA. These three molecules compete for the binding to type 1 IL1 receptor (IL-1R1). IL1α is produced as a pro-protein by macrophages and monocytes, and is processed to its active form as a response to cell injury, inducing cell apoptosis. IL1α gene polymorphism has been associated with Alzheimer’s disease and rheumatoid arthritis.
|Assay Target Class||Cytokine|
|Experimental Type||In vitro|
|Product Brand Name||LANCE Ultra|
|Shipping Condition||Blue Ice|
|Unit Size||500 assay points|
The introduction of enzyme-linked immunosorbent assays (ELISAs) in the early 1970’s offered researchers a non-radiometric immunoassay platform without compromising sensitivity. Over the last 50 years scientists have made huge strides in disease research and drug discovery and a demand for greater assay throughput and sensitivity has evolved. In response, more robust immunoassays have been developed to address some of the limitations of the standard, colorimetric ELISA.
Find out about the most common limitations of traditional ELISAs and how different ELISA alternative technologies address these limitations.