For research use only. Not for use in diagnostic procedures.
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Attention: Glucagon Analyte included in this kit is provided in solution. Please store at -20°C upon receipt of the kit. Upon the first use of the kit aliquot the analyte and store the aliquots at -20°C to avoid further freeze/thaw cycles.
The LANCE® Ultra Human Glucagon Detection Kit is designed for detection and quantitation of human glucagon in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.
Glucagon is a 29 amino acid peptide hormone produced by the pancreas. Glucagon generally functions as a counterregulatory homrone opposing the actions of insulin to maintain appropriate levels of blood glucose. Normal human serum glucagon levels range from 50-200 pg/mL. The glucagon:insulin ratio controls the rate of gluconeogenesis and glycogenolysis, disruption of this ratio can have severe metabolic implications. The present kit permits detection of glucagon (i.e. analyte) in different sample matrices.
|Assay Target Class||Hormone|
|Detection Method||Time-Resolved Fluorescence (TRF), TR-FRET|
|Experimental Type||In vitro|
|Product Brand Name||LANCE Ultra|
|Shipping Condition||Blue Ice|
|Unit Size||500 Assay Points|