For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
The LANCE® Ultra Human Adiponectin Detection Kit is designed for detection and quantitation of human adiponectin in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.
Adiponectin (Acrp30, in mouse) is a hormone secreted by differentiated adipocytes that regulates energy homeostasis and glucose and lipid metabolism. It is expressed as a ~35 kDa protein. It has been demonstrated that Adiponectin / Acrp30 plays a major role in controlling whole-body metabolism, particularly by enhancing insulin sensitivity in muscle and liver, and by augmenting the oxydation of fatty acids in muscle.
| Assay Target | Adiponectin |
|---|---|
| Assay Target Class | Protein |
| Automation Compatible | Yes |
| Detection Method | Time-Resolved Fluorescence (TRF), TR-FRET |
| Experimental Type | In vitro |
| Product Brand Name | LANCE Ultra |
| Shipping Condition | Blue Ice |
| Therapeutic Area | Metabolic |
| Unit Size | 10,000 Assay Points |
