Phospho-ERK 1/2 (Thr202/Tyr204) LANCE Ultra TR-FRET Cellular Detection Kit, 500 Assay Points

Please note control lysates are NOT included in the kits. Control lysates are sold separately, catalog number TRF4000S.

Formats:

• Our 500 assay point kit allows you to run 500 wells in 384-well format, using a 20 µL reaction volume.
• Our 10,000 assay point kit allows you to run 10,000 wells in 384-well format, using a 20 µL reaction volume.

LANCE^® and LANCE^® (Lanthanide chelate excite) Ultra are homogeneous (no wash) TR-FRET (time-resolved fluorescence resonance energy transfer) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second antibody is labeled with an acceptor fluorophore [ULight™ dye]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm. Data are represented as ratiometric (665/615 nm X 10,000).

ERK1 and ERK2, also known as MAP Kinase 3 and MAP Kinase 1, are serine/threonine kinases and important targets for cancer treatment. ERK kinases are dually phosphorylated at Tyr-202 and Tyr-204, leading to activation of the target. ERK can also be dephosphorylated at Tyr-202 by PTPRJ. A number of downstream targets have been identified including EIF4EBP1, which is necessary for the initiation of translation. It has also been shown to phosphorylate ELK-1.