LANCE Ultra phospho-ERK 1/2 (Thr202/Tyr204) kits are designed for the detection of phosphorylated ERK 1 and ERK2 in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
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For research use only. Not for use in diagnostic procedures.
Please note control lysates are NOT included in the kits. Control lysates are sold separately, catalog number TRF4000S.
Formats:
LANCE® and LANCE® (Lanthanide chelate excite) Ultra are homogeneous (no wash) TR-FRET (time-resolved fluorescence resonance energy transfer) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second antibody is labeled with an acceptor fluorophore [ULight™ dye]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm. Data are represented as ratiometric (665/615 nm X 10,000).
ERK1 and ERK2, also known as MAP Kinase 3 and MAP Kinase 1, are serine/threonine kinases and important targets for cancer treatment. ERK kinases are dually phosphorylated at Tyr-202 and Tyr-204, leading to activation of the target. ERK can also be dephosphorylated at Tyr-202 by PTPRJ. A number of downstream targets have been identified including EIF4EBP1, which is necessary for the initiation of translation. It has also been shown to phosphorylate ELK-1.
| Assay Pathway | MAPK |
|---|---|
| Assay Target | ERK1/2 |
| Assay Target Class | Phosphoprotein |
| Automation Compatible | Yes |
| Detection Method | Time-Resolved Fluorescence (TRF), TR-FRET |
| Product Brand Name | LANCE Ultra |
| Shipping Condition | Blue Ice |
| Target Species | Human |
| Unit Size | 10,000 Assay Points |
