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Chorionic gonadotropin (human) LANCE Ultra TR-FRET Detection Kit, 10,000 Assay Points

The LANCE® Ultra Human Chorionic Gonadotropin (hCG) Detection Kit is designed for detection and quantitation of hCG in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

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For research use only. Not for use in diagnostic procedures.

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TRF3001C
500 Assay Points
773.00 USD
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TRF3001M
10,000 Assay Points
10300.00 USD
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Detail Information

The LANCE® Ultra Human Chorionic Gonadotropin (hCG) Detection Kit is designed for detection and quantitation of hCG in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

  • No-wash steps, no separation steps
  • TR-FRET technology
  • Sensitive detection
  • High reproducibility
  • Faster time-to-results
  • Easy automation
  • 96-well, 384-well, and 1536-well formats

LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.

Human Chorionic Gonadotropin (hCG) is a glycoprotein hormone typically detected in pregnancy tests but can also be produced by some cancerous tumors. It is heterodimeric in which the alpha subunit is shared amongst other similar glycoproteins. The beta subunit is unique to hCG. hCG interacts with the LHCG receptor in order to promote maintenance of the corpeus luteum at the beginning stages of pregnancy. hCG is produced by the syncytiotrophoblast in the placenta. The beta subunit is excreted by various cancers such as seminoma and germ cell tumors.

Specifications

Assay Target hCG
Assay Target Class Hormone
Automation Compatible Yes
Detection Method Time-Resolved Fluorescence (TRF), TR-FRET
Experimental Type In vitro
Product Brand Name LANCE Ultra
Shipping Condition Blue Ice
Target Species Human
Therapeutic Area Metabolic
Unit Size 10,000 Assay Points
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Brochure

Manual

LANCE Ultra Human Chorionic Gonadotropin (hCG) Detection Kit TRF3001

This manual explains how to run a LANCE Ultra TR-FRET detection assay for human chorionic gonadotrop

PDF 516 KB