The LANCE® Ultra cAMP assay is a homogeneous time-resolved fluorescence resonance energy transfer (TR-FRET) immunoassay designed to measure cAMP produced upon modulation of adenylyl cyclase activity by G-protein coupled receptors (GPCRs).
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PerkinElmer's LANCE® Ultra cAMP assay offers unmatched sensitivity and S/B using a simple TR-FRET protocol. Ideal for any Gs- or Gi-coupled GPCR assay, this Ultra-sensitive cAMP assay is supreme when working with low cell numbers, endogenous receptors, or difficult Gi-coupled receptors.
Features:
The LANCE Ultra cAMP assay is a homogeneous time-resolved fluorescence resonance energy transfer (TR-FRET) immunoassay designed to measure cAMP produced upon modulation of adenylyl cyclase activity by G-protein coupled receptors (GPCRs).
The assay is based on the competition between the europium (Eu) chelate-labeled cAMP tracer and cellular cAMP for binding sites on cAMP-specific monoclonal antibodies labeled with the ULight™ dye. When antibodies are bound to the Eu-labeled cAMP tracer, light pulse at 320 or 340 nm excites the Eu chelate molecule of the tracer. The energy emitted by the excited Eu chelate is transferred by FRET to ULight molecules on the antibodies, which in turn emit light at 665 nm.
| Assay Target | cAMP |
|---|---|
| Automation Compatible | Yes |
| Detection Method | Time-Resolved Fluorescence (TRF), TR-FRET |
| Experimental Type | In vitro |
| Product Brand Name | LANCE Ultra |
| Second Messenger Release | cAMP |
| Shipping Condition | Blue Ice |
| Unit Size | 1,000 assay points |
Cyclic nucleotide phosphodiesterases (PDEs) catalyze the hydrolysis of 3',5'-cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP) to their corresponding 5’-nucleotide monophosphates, AMP and GMP.
Guanosine triphosphate binding protein coupled receptors (GPCRs) represent one of the largest and most important classes of pharmaceutical drug targets. Approximately 48% of all GPCRs couple through adenylate cyclase making the need for a robust cAMP detection method critical.
The LANCE® Ultra cAMP kit is intended for the quantitative determination of 3’,5’-cyclic adenosine monophosphate (cAMP) in cell lysate and cellular membrane samples.
The LANCE® Ultra cAMP assay is a second-generation LANCE time-resolved fluorescence resonance energy transfer (TR-FRET) immunoassay designed to measure cAMP produced upon modulation of adenylyl cyclase activity by activated guanosine triphosphate binding protein-coupled receptors (GPCRs). The homogeneous two-component assay is based on the competition between europium chelate-labeled cAMP and cellular cAMP for binding to high-affinityanti-cAMP monoclonal antibodies labeled with the ULight™ dye.
Poster on High Sensitivity cAMP Assays in Primary Cells and Mesenchymal Stem Cells with the LANCE® Ultra cAMP kit
Guanosine triphosphate binding protein-coupled receptors (GPCRs) represent one of the largest and most important classes of pharmaceutical drug targets. We have developed a second generation LANCE® time-resolved fluorescence resonance energy transfer (TR-FRET) immunoassay designed to measure cAMP produced upon modulation of adenylyl cyclase activity by activated GPCRs.
