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The LANCE Ultra c-Myc Detection Kit is designed for detection and quantitation of c-Myc peptide tag in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
LANCE and LANCE (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with an acceptor fluorophore [ULight™ dye]. A “tracer” analyte is biotinylated and binds Streptavidin labeled with donor Eu-chelate. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm. In the presence of sufficient non-biotinylated analyte this emission will be diminished.
The c-myc polypeptide tag is derived from the c-myc gene. The tag can be used during affinity chromatography and to separate wild-type protein from recombinant protein. The c-myc tag can be used in indirect detection assays for which there is no antibody for the target.
|Assay Target Class||Peptide|
|Detection Method||Time-Resolved Fluorescence (TRF), TR-FRET|
|Product Brand Name||LANCE Ultra|
|Shipping Condition||Blue Ice|
|Unit Size||500 Assay Points|
This manual explains how to run the LANCE Ultra TR-FRET c-Myc detection assay.