For research use only. Not for use in diagnostic procedures.
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The LANCE® Ultra Human Apo-CIII Detection Kit is designed for detection and quantitation of ApoC3 in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.
Apolipoprotein CIII (ApoCIII), a relatively small protein (79 amino acids), is a component of very low density lipoproteins. Apo-CIII delays the catabolism of triglyceride rich particles by inhibiting the activities of lipoprotein lipase, hepatic lipase, and blocks the uptake of triglyceride-rich particles by the cells. Therefore, ApoCIII has been associated with coronary heart disease and diabetes. An increase in ApoCIII levels induces the development of hypertriglyceridemia and associated with non-alcoholic fatty liver disease.
|Assay Target Class||Protein|
|Detection Method||Time-Resolved Fluorescence (TRF), TR-FRET|
|Experimental Type||In vitro|
|Product Brand Name||LANCE Ultra|
|Shipping Condition||Blue Ice|
|Unit Size||500 Assay Points|
This manual explains how to run the LANCE Ultra TR-FRET human Apo-CIII detection assay.