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ANGPT1 (human) LANCE Ultra TR-FRET Detection Kit, 500 Assay Points

The LANCE® Ultra Human ANGPT1 Detection Kit is designed for detection and quantitation of human angiopoietin 1 in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

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For research use only. Not for use in diagnostic procedures.

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TRF3014C
500 Assay Points
796.00 USD
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10,000 Assay Points
10600.00 USD
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Detail Information

The LANCE® Ultra Human ANGPT1 Detection Kit is designed for detection and quantitation of human angiopoietin 1 (ANGPT1) in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

  • No-wash steps, no separation steps
  • TR-FRET technology
  • Sensitive detection
  • High reproducibility
  • Faster time-to-results
  • Easy automation
  • 96-well, 384-well, and 1536-well formats

LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.

Angiopoietin belongs to a family of vascular growth factors. There are several angiopoietins: ANGPT1, ANGPT2, ANGPT4 now identified, and a number of closely related proteins: ANGPTL1, ANGPTL2, ANGPTL3, ANGPTL4, ANGPTL5, ANGPTL6, and ANGPTL7. Angiopoietin 1(Ang-1; also ANGPT1) is a 70 kDa glycoprotein which contains 498 amino acids, including a coiled-coiled domain near the amino-terminus and a fibrinogen-like domain at the C-terminus. It plays a critical role in vessel maturation, adhesion, migration, and survival.

Specifications

Assay Target ANGPT1
Assay Target Class Protein
Automation Compatible Yes
Detection Method Time-Resolved Fluorescence (TRF), TR-FRET
Product Brand Name LANCE Ultra
Shipping Condition Blue Ice
Target Species Human
Therapeutic Area Angiogenesis
Unit Size 500 Assay Points
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Manual

Manual: LANCE Human ANGPT1 Detection Kit TRF3014

This manual explains how to run the LANCE Ultra TR-FRET human ANGPT1 detection assay.

PDF 535 KB