Europium-labeled anti-GST antibody, for capturing GST-tagged proteins in LANCE® TR-FRET assays.
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The anti-GST antibody is purified from goat serum. The anti-GST antibody binds to GST protein from Schistosoma japonicum expressed from the pGEX vector in E.coli.
LANCE® (Lanthanide chelate excite) and LANCE® Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One molecule of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore [ULight™ dye, allophycocyanin (APC), etc.]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm (for ULight dye and APC).
|Detection Method||Time-Resolved Fluorescence (TRF), TR-FRET|
|Experimental Type||In vitro|
|Product Brand Name||LANCE|
|Shipping Condition||Blue Ice|
|Unit Size||50 µg|
This data sheet describes LANCETM Normalization using Wallac brand instruments. LANCE normalization can be performed either by using WIZARD software, which will guides us through the process or the Normalization samples which are run among other samples i
Generic LANCE reagents are intended for setting up TR-FRET based assays using Eu-chelate label as a donor and APC-labeled reagent as an acceptor.