LANCE® Eu-labeled anti-c-myc antibodies are purified mouse IgG1 monoclonal antibodies, which recognize the epitope sequence (EQKLISEEDL) derived from the human c-myc protein. These monoclonal antibodies against the c-myc epitope do not cross react with other cellular proteins.
LANCE® (Lanthanide chelate excite) and LANCE® Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One molecule of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore [ULight™ dye, allophycocyanin (APC), etc.]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm (for ULight dye and APC).
| Antibody Conjugates | Anti-c-Myc |
|---|---|
| Automation Compatible | Yes |
| Detection Method | Time-Resolved Fluorescence (TRF), TR-FRET |
| Experimental Type | In vitro |
| Fluorophore | Eu-W1024 |
| Product Brand Name | LANCE |
| Shipping Condition | Dry Ice |
| Unit Size | 10 µg |
Generic LANCE reagents are intended for setting up TR-FRET based assays using Eu-chelate label as a donor and APC-labeled reagent as an acceptor.
