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LANCE cAMP Detection Kit, 10,000 assay points

LANCE® TR-FRET cAMP assay kit, for Gs- and Gi-coupled GPCR functional assays.

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AD0262
500 Assay Points
167.00 USD
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AD0262E
1,000 Assay Points
540.00 USD
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AD0263
10,000 Assay Points
1676.00 USD
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AD0264
50,000 Assay Points
7400.00 USD
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Detail Information

LANCE cAMP Assay. Red, robust and ready for uHTS! Measure cAMP produced by whole cells or membrane receptors stimulated with GPCR agonists and antagonists with more sensitivity and precision than ever before and outstanding productivity.

PerkinElmer's innovative LANCE® cAMP assay combines homogeneous TR-FRET technology with red-shifted Alexa® Fluor dye chemistry for maximum excitation/emission discrimination. The result is a FRET assay without the compound interference associated with blue dyes. You can read longer without accumulating background signal to maximize S/N and Z'.

Features:

  • High sensitivity: fmol detection, leading to improved performance and productivity
  • High precision: Z' = 0.8 for Gai cell assay, 0.8 for Gas cell assay
  • Flexible sample formats: work with whole cells or membranes
  • Easy to automate: simple one-step protocol (following cell stimulation)
  • Ultra HTS-compatible: simple conversion between 96-, 384- or 1536-well plate formats results in dramatic savings
  • Stable signal (>20 hours at room temperature): ideal for screening
  • Read on any TRF-compatible instrument, such as PerkinElmer's ViewLux, EnVision, VICTOR3 and Fusion Readers

LANCE cAMP Assay Principle. Light pulse at 340 nm excites the Europium-chelate of the Eu-SA/b-cAMP tracer. The energy emitted from the chelate is transferred to the Alexa-labeled antibodies bound to the tracer, generating a TR-FRET signal at 665 nm. Residual energy from the chelate will produce light at 615 nm. cAMP of a sample competes with the tracer for antibody binding sites and causes signal reduction. This superior performance and ultra high throughput screening convenience leads to dramatic cost savings.

Specifications

Assay Target cAMP
Automation Compatible Yes
Detection Method Time-Resolved Fluorescence (TRF), TR-FRET
Experimental Type In vitro
Product Brand Name LANCE
Quantity in a Package Amount 10000.0 Units
Second Messenger Release cAMP
Shipping Condition Blue Ice
Unit Size 10,000 Assay Points
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Application Note

A Comparison of PerkinElmer's LANCE cAMP Assay Performance to that of State of the Art Competitive Technologies

G-protein coupled receptors(GPCRs) continue to represent the most important class of pharmaceutical targets. Their activity is commonly assessed by measuring the intracellular second messengers released upon their stimulationby appropriate agonists. Cyclic AMP (cAMP) is one of thesecond messengers frequently measured to find and characterize both agonist and antagonist drug leads.

PDF 655 KB
Quench Correction for LANCE Time-Resolved Fluorescence Resonance Energy Transfer

This data sheet describes LANCETM Normalization using Wallac brand instruments. LANCE normalization can be performed either by using WIZARD software, which will guides us through the process or the Normalization samples which are run among other samples i

PDF 847 KB

Brochure

Data Sheet

LANCE Controls - For Service Purposes Only - AD0163

LANCE Controls are intended for in vitro use for testing instrument performance in LANCE measurements.

PDF 231 KB

Manual

LANCE™ cAMP 384 Kit

This LANCE™ cAMP 384 kit is intended for the quantitative determination of adenosine 3',5'-cyclic monophosphate (cAMP) in cell culture and cell membrane samples.

PDF 244 KB