Label antibodies, small molecules, proteins, or peptides with IVISense NIR fluorescent dyes.
PerkinElmer’s IVISense™ fluorescent dyes (VivoTag®) for labeling antibodies, small molecules, proteins, or peptides are designed specifically to enable customized development of novel superbright fluorescent imaging agents. Dye properties, such as excellent brightness, hydrolytic stability, and photostability, as well as selection for minimal interaction with biological tissue for no potential loss of specificity, have been optimized and validated for use in in vivo or in vitro imaging.
Available as NHS esters or maleimide reactive dyes for conjugation to either free amine (-NH3) or free thiol (-SH) containing molecules. Conjugate an optimal 1-3 dyes onto your protein or choose self-quenching dyes to make your own custom fluorogenic probes.
For research use only. Not for use in diagnostic procedures.
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IVISense 750 NHS fluorescent dye is an amine-reactive near-infrared (NIR) fluorochrome for coupling via an N-Hydroxysuccinimide (NHS)-ester linkage to peptides, small molecules, proteins, antibodies or macromolecules. Unit size 5 mg.
|IVISense NHS FLUORESCENT DYES|
|IVISense 645 NHS||643/660||Suitable for labeling free amines (typically lysine residues)|
|IVISense 680 NHS||665/688|
|IVISense 800 NHS||785/810|
|IVISense MAL FLUORESCENT DYES|
|IVISense 645 MAL||643/660||Suitable for labeling free thiols (typically cysteine residues)|
|IVISense 680 MAL||665/688|
|IVISense SELF-QUENCHING DYES|
|IVISense 680 NHS||673/691||When a protein is labeled with multiple IVISense Self-Quenching Dye molecules, there is a shift in the absorbance of the dye, which will reduce the signal. Only choose IVISense Self-Quenching Dyes if your labeling ratio of dye: protein will be 1:1. If multiple dyes are to be conjugated onto your protein, a non-self-quenching IVISense Dye should be used. NHS dyes are suitable for labeling free amines (typically lysine residues), while MAL dyes are suitable for labeling free thiols (typically cysteine residues).|
|IVISense 750 NHS||750/775|
|IVISense 750 MAL||750/775|
|Optical Imaging Classification||Fluorescence Imaging|
|Product Brand Name||IVISense|
|Quantity in a Package Amount||1.0 Units|
|Shipping Condition||Blue Ice|
|Unit Size||5 mg|
|Wave Length||750 nm|
N-Hydroxysuccinimide (NHS)-ester labeling reagents, such as VivoTag®-S reagents, are commonly used for labeling proteins. The NHS ester moiety reacts with amino groups at pH 7-9 to form stable amide bonds. Lysines within proteins, including antibodies, are available as targets for this chemical conjugation.,Protein labeling efficiency may vary depending upon the type of protein labeled, so different conjugation ratios may need to be attempted to attain success. For Near Infrared (NIR) fluorophore conjugation, ratios of fluorophore to protein of 4:1 have been seen to generate effective imaging agents. Not all antibodies or proteins (independent of target specificity) make good imaging agents, due to long half-lives and/or excessive accumulation in non-target sites.
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