PerkinElmer
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IVISbrite Red F-luc-Puromycin Lentiviral Particles (RediFect)

Create the bioluminescent cell line you need

Stably-transduce cells with prepackaged IVISbrite Lentiviral Particles (RediFect Lentiviral Particles) expressing a combination of novel luciferases and a fluorescent protein for monitoring tumor growth, tracking primary or stem cells in vivo, and other applications.

PerkinElmer’s IVISbrite lentiviral particles contain Red F-luc, the same red-shifted firefly luciferase (Luciola Italica) as our IVISbrite tumor cell lines, for a stable bioluminescent signal excellent for deep tissue imaging; or use our green-shifted Renilla luciferase (Renilla reniformis) transgene. Both offer efficient transduction of a wide variety of mammalian cell lines including most cancer cell lines, primary, stem and non-dividing cells. Bioluminescent cells can be imaged using our luciferin or coelenterazine substrates.

For research use only. Not for use in diagnostic procedures.

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SKU Display Name
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CLS960002
IVISbrite Red F-luc-Puromycin Lentiviral Particles (RediFect)
1710.00 USD
 
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CLS960003
IVISbrite Red F-luc-GFP Lentiviral Particles (RediFect)
1710.00 USD
 
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CLS960004
IVISbrite Green Renilla-Puromycin Lentiviral Particles (RediFect)
1714.00 USD
 
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Overview

IVISbrite Red F-luc-Puromycin Lentiviral Particles are self-inactivating, recombination incompetent lentiviral particles carrying red-shifted Luciola Italica luciferase transgene under control of the stable UbC promoter. The luciferase transgene is fused to the puromycin resistance gene via T2A “self-cleaving” linker peptide for efficient co-expression with selection marker. The lentiviral particles are pseudotyped with G glycoprotein from Vesicular Stomatitis Virus (VSVG), allowing efficient transduction of a wide variety of mammalian cells including most cancer cell lines, primary, stem and non-dividing cells.

 

  • Each vial contains 1 x 107 units/ml of lentiviral particles resuspended in 200 ul
  • The packaged material provides sufficient number of lentiviral particles for transduction of at least one cell line

We recommend using this product to transduce human cell lines, or murine cell lines that will be used in Nude and SCID mouse models only. An immune response may occur in syngeneic mouse models.

Specifications

Optical Imaging Classification Bioluminescence Imaging
Product Brand Name IVISbrite
Shipping Condition Dry Ice
Type Lentiviral particles
Unit Size 1 Vial
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