Create the bioluminescent cell line you need
Stably-transduce cells with prepackaged IVISbrite™ Lentiviral Particles (RediFect™ Lentiviral Particles) expressing a combination of novel luciferases and a fluorescent protein for monitoring tumor growth, tracking primary or stem cells in vivo, and other applications.
PerkinElmer’s IVISbrite lentiviral particles contain Red F-luc, the same red-shifted firefly luciferase (Luciola Italica) as our IVISbrite tumor cell lines, for a stable bioluminescent signal excellent for deep tissue imaging; or use our green-shifted Renilla luciferase (Renilla reniformis) transgene. Both offer efficient transduction of a wide variety of mammalian cell lines including most cancer cell lines, primary, stem and non-dividing cells. Bioluminescent cells can be imaged using our luciferin or coelenterazine substrates.
For research use only. Not for use in diagnostic procedures.
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IVISbrite Green Renilla-Puromycin Lentiviral Particles are self-inactivating, recombination incompetent lentiviral particles carrying green emitting Renilla luciferase transgene under control of the stable UbC promoter. The luciferase transgene is fused to the puromycin resistance gene via T2A “self-cleaving” linker peptide for efficient coexpression with selection marker. The lentiviral particles are pseudotyped with G glycoprotein from Vesicular Stomatitis Virus (VSVG), allowing efficient transduction of a wide variety of mammalian cells including most cancer cell lines, primary, stem and non-dividing cells.
We recommend using this product to transduce human cell lines, or murine cell lines that will be used in Nude and SCID mouse models only. An immune response may occur in syngeneic mouse models.
|Optical Imaging Classification||Bioluminescence Imaging|
|Product Brand Name||IVISbrite|
|Shipping Condition||Dry Ice|
|Unit Size||1 Vial|
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