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IVIS Lumina XRMS In Vivo Imaging System

Industry leading Fluorescence, Bioluminescence and X-Ray Imaging - All-In-One benchtop instrument! The IVIS® Lumina XRMS (x-ray multi-species optical imaging system) adds to the versatility of the IVIS Lumina XR by offering the flexibility to image large animals up to 500 grams with precise optical and X-Ray overlay.

Part Number CLS136340
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For research use only. Not for use in diagnostic procedures.

Overview

The Lumina XRMS includes state of the art spectral unmixing for sensitive multispectral imaging to monitor multiple biological events in the same animal. Use our Living Image® software to automate all the controls and settings required for seamless image acquisition and processing. Typical X-ray image acquisitions take only 10 seconds and can be overlaid with both optical and photographic images.

Superior Optical Imaging with Spectral Unmixing

The IVIS Lumina XRMS Series III is capable of imaging all common fluorescent and bioluminescent reporters or dyes. The system is equipped with up to 21 filter sets to image reporters that emit from green to near-infrared. High resolution, sharp cut-off filters are simply interchangeable to achieve the highest performance, sensitivity and spectral unmixing. The Lumina XRMS imaging system also accommodates Petri dishes or micro-titer plates for in vitro imaging.

 

The system can incorporate premium animal handling features such as a heated stage, gas anesthesia connections and a syringe injection system for simultaneous compound administration. Living Image software yields high-quality, reproducible, quantitative results incorporating instrument calibration, background subtraction and the image algorithms. Simple user guided spectral unmixing allows detection and separation of multiple reporters, and Living Image provides the precise overlay to see your optical reporters together with anatomical surface or X-ray features.

Features and Benefits:

  • Multispecies optical and X-ray imaging
  • Image mice, rats and other large animals
  • High resolution, low dose digital X-Ray
  • Exquisite sensitivity in bioluminescence
  • Compute Pure Spectrum (CPS) spectral unmixing for ultimate fluorescence sensitivity
  • Full fluorescence tunability through the NIR Spectrum

Selected Publications

  • Zhang et al (2021). One-step and facile synthesis of peptide-like poly(melphalan) nanodrug for cancer therapy. Nanotoday. 37; 101098. https://doi.org/10.1016/j.nantod.2021.101098
  • Zou et al (2020). A ZIF-90 nanoplatform loaded with an enzyme-responsive organic small-molecule probe for imaging the hypoxia status of tumor cells. Nanoscale. 12, 14870-14881. https://doi.org/10.1039/D0NR02580A
  • Parkins et al (2020). Engineering Circulating Tumor Cells as Novel Cancer Theranostics. Theranostics. 10(17): 7925–7937. https://dx.doi.org/10.7150%2Fthno.44259
  • Son et al (2019). Gelatin–chlorin e6 conjugate for in vivo photodynamic therapy. J Nanobiotechnology. 17(50). https://doi.org/10.1186/s12951-019-0475-1
  • Wang et al (2019). A Y1 receptor ligand synergized with a P-glycoprotein inhibitor improves the therapeutic efficacy of multidrug resistant breast cancer. Biomater. Sci., 7, 4748-4757.https://doi.org/10.1039/C9BM00337A
  • Gao et al (2018). A unique off-on near-infrared cyanine-based probe for imaging of endogenous alkaline phosphatase activity in cells and in vivo. Sensors & Actuators: Chemical. 265: 565-574. https://doi.org/10.1016/j.snb.2018.03.078
  • Shen et al (2018). Versatile hyaluronic acid modified AQ4N-Cu(II)-gossypol infinite coordination polymer nanoparticles: Multiple tumor targeting, highly efficient synergistic chemotherapy, and real-time self-monitoring. Biomaterials. 154: 197-212. https://doi.org/10.1016/j.biomaterials.2017.11.001
  • Gong et al (2018). A Ultrasensitive Near‐Infrared Fluorescent Probe Reveals Pyroglutamate Aminopeptidase 1 Can Be a New Inflammatory Cytokine. Adv Scihttps://doi.org/10.1002/advs.201700664
  • Lai et al (2016). Fluorescent gold nanoclusters for in vivo target imaging of Alzheimer's disease. RSC Advances. 6, 30081-30088. https://doi.org/10.1039/C6RA01027J
  • Zhao et al (2016). In vivo target bio-imaging of cerebral ischemic stroke by real-time labeling of zinc. RSC Adv., 2016,6, 110525-110534. https://doi.org/10.1039/C6RA23507G

For additional publications, please visit Google Scholar.

Specifications

Height 104.0 cm
Imaging Modality Optical Imaging
Optical Imaging Classification Bioluminescence imaging, Fluorescence Imaging
Portable No
Product Brand Name IVIS
Width 48.0 cm
Resources, Events & More
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Application Note

Cerenkov Imaging of Radioisotopes in IVIS systems

Cerenkov Emission from radioisotopes in tissue,Optical imaging detects photons in the visible range of the electromagnetic,spectrum. PET and SPECT imaging instruments are sensitive to photons in the much,higher energy range of x-rays and gamma rays. While the PET and SPECT probes,which can generate Cerenkov light in tissue will continue to produce the relevant,gamma- and x-rays, visible photons will be produced from the Cerenkov emission,which the IVIS® will detect.,In beta decay emitters such as PET probes and isotopes such as 90Y, 177Lu, 131I and 32P,the beta particle will travel in the tissue until it either annihilates with an electron or,loses momentum due to viscous electromagnetic forces.,It is possible that the beta (electron or positron),is relativistic, traveling faster than the speed,of light in the tissue. While it is impossible,to travel than the speed of light in a vacuum,(c), the speed of light in tissue is v = c / n,where n is the tissue index of refraction and,n = 1. Cerenkov photons will be generated,by a relativistic charged particle in a dielectric medium such as tissue.

PDF 2 MB
Imaging Oncolytic Virus Infection in Cancer Cells

Aside from the traditional small-molecule chemotherapeutics or targeted therapy agents that have been widely used in the clinic for decades, a new type of cancer therapeutics based on oncolytic viruses has recently gained attention in the field of research. Oncolytic viruses are genetically modified viruses capable of delivering therapeutic gene payload to cancer cells.

There are many types of oncolytic viruses each having a different tumor-targeting mechanism. This application note highlights using Sindbis pseudovirus genetically modified with firefly luciferase reporter gene to non-invasively evaluate, monitor, and quantify oncolytic viral infection in living tumors and subsequent virus-host interactions in real-time using IVIS® optical imaging.

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Stem Cell Research and Regenerative Medicine

With the potential to treat a wide range of disease, from organ damage to congenital defects, stem cell research and tissue engineering form the underlying basis of regenerative medicine. Significant advances in the science of skin regeneration, for example, have now made it possible to develop and grow artificial skin grafts in a lab for treatment of burn victims. Other therapeutic applications include the use of stem cells to treat and repair central nervous system diseases such as ischemia and cerebral palsy, cardiovascular diseases, as well as autoimmune diseases including type I diabetes.

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Case Study

Tracking Neuroinflammation Using Transgenic Mouse Models and Optical Imaging

Amyotrophic lateral sclerosis (ALS) is a devastating neurological disease for which there is no cure. Another lethal brain disease is stroke, which occurs when blood supply to the brain is disrupted by a blood vessel that bursts or becomes blocked. Neuroinflammation plays a key role in both of these diseases as well as in the pathogenesis for Alzheimer’s, multiple sclerosis, and other forms of brain injury.

Read this case study where Dr. Jasna Kriz and her colleagues at Laval University in Quebec, developed transgenic mouse models for optical imaging of cells such as astroglia, microglia, and neurons. Mice were modified to express firefly luciferase when immune processes are activated enabling the researchers to study early neuroinflammatory events using the high sensitivity 2D & 3D optical imaging on the IVIS® platform.

PDF 1 MB
A Novel Mouse Model Using Optical Imaging to Detect On-Target, Off-Tumor CAR-T Cell Toxicity

CAR T therapy has achieved tremendous success in treating blood malignancies, however treating solid tumors with this therapy has proven to be challenging due to several factors such as on-tumor, off-tumor toxicity.

Read this case study where researchers from University of Pennsylvania created a mouse model that expresses tumor associated antigens in normal tissue to study off-tumor CAR-T cell toxicity. These studies used optical imaging on the IVIS® platform to longitudinally monitor off-tumor antigen expression, tumor progression, and CAR-T cell trafficking in live animals.

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A Novel Non-Invasive In Vivo Diagnostic Tool for the Assessment of NASH

Non-alcoholic fatty liver disease (NAFLD) describes a progressive pathology that affects the liver. Fat accumulation causes fatty liver (NAFL) or steatosis to develop, which leads to lipotoxicity and in turn induces liver inflammation and apoptosis, resulting in non-alcoholic steatohepatitis (NASH). NASH can progress to fibrosis and then cirrhosis, which in some cases will lead to hepatocellular carcinoma (HCC).

Read this case study to learn how non-invasive preclinical in vivo imaging was used to longitudinally visualize, quantify, and diagnose NASH with the goal of investigating the efficacy of liver fibrosis-preventing drugs on NAFLD progression.

PDF 963 KB
Using IVIS optical imaging of CRISPR/Cas9 engineered adipose tissue to study obesity prevention

Obesity is a global epidemic that is the fifth leading cause of death worldwide and in the US alone, nearly 85% of adults are expected to be overweight or obese by 2030. In addition to the increased risk of overall mortality, obesity is associated with an increased risk for other metabolic disorders such as diabetes and heart disease which pose a significant burden to health care systems.

Read this case study to learn how researchers at the Joslin Diabetes Center in Boston, Massachusetts used the IVIS® optical imaging system to visualize and quantify CRISPR/Cas9 engineered adipose tissue in a mouse model to study the prevention of obesity and obesity-related metabolic disorders.

PDF 1 MB

Ebook

In Vivo Imaging Solutions eBook

Researchers trust our in vivo imaging solutions to give them reliable, calibrated data that reveals pathway characterization and therapeutic efficacies for a broad range of indications. Our reagents, instruments, and applications support have helped hundreds of research projects over the years. And our hard-earned expertise makes us a trusted provider of pre-clinical imaging solutions— with more than 9,000 peer reviewed articles as proof.

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Featured Publication Note

In Vivo Imaging of Influenza Virus Infection in Immunized Mice

Influenza is a highly infectious airborne disease with an important societal burden. Annual epidemics have occurred throughout history causing tens of millions of deaths. Even a run-of-the-mill influenza infection can be debilitating to otherwise healthy people, and lethal to those who are elderly or frail, so vaccinations are important. Because of seasonal antigenic drift and antiviral resistance of the virus there is a critical need for the development of new and novel vaccines and antiviral drugs. In vivo optical imaging has emerged as a powerful, non-invasive tool to track viral load and therapeutic efficacy of vaccines and immunotherapies in small animal models.

Read how researchers at the NIH, NIAID, Emory University, and University of Wisconsin used the IVIS® optical imaging platform to successfully quantify and track viral load in mice and demonstrated that vaccine of human mAb administration has a protective or therapeutic effect in mice challenged with the influenza virus.

PDF 436 KB
Evaluating a Novel Nanoparticle Platform for Controlled Liraglutide Release in a Type II Diabetes Mouse Model Via Optical Imaging

A large percentage of Type 2 diabetes mortality is related to cardiovascular complications. Consequently, there is a critical need for creating novel therapeutics that not only manage blood glucose levels, but also reduce the risk of developing cardiovascular diseases.

Liraglutide (Lira) is a recently approved drug used to treat Type II diabetes with excellent hypoglycemic effects while also improving cardiovascular function in patients. However its short half-life requires daily injections increasing the risk of poor patient compliance and other complications.

Read this publication review to learn how researchers used a Type II diabetes mouse model and optical imaging with the IVIS® platform to evaluate a nanoparticle system that offers a sustained and controlled release of Lira that overcomes the challenges of the short half-life of the drug.

PDF 538 KB

Flyer

Best Practices for Designing An Effective In Vivo Fluorescence Imaging Study

Fluorescence molecular imaging is the visualization of cellular and biological function in vivo to gain deeper insights into disease processes and treatment effects. Designing an effective study from the beginning can help save time and resources.

Learn about several important best practices, from proper probe selection to study design to imaging technique tips and tricks needed to generate meaningful biological information from your in vivo fluorescence imaging studies.

PDF 507 KB

Product Note

IVIS Lumina XRMS Series III Product Note

IVIS Lumina XR Series III Integrating Gold Standard Bioluminescence, Fluorescence and X-Ray In Vivo Technologies. The IVIS Lumina XR Series III from PerkinElmer provides an expandable, sensitive imaging system that is easy to use for fluorescent, bioluminescent, radioistopic and X-Ray imaging in vivo. As the leading optical imaging platform for in vivo analysis, IVIS systems include a range of practical accessories developed through experience in research laboratories worldwide.

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Software Downloads

Technical Note

Adaptive Fluorescence Background Subtraction

Adaptive Fluorescence Background Subtraction Pre-clinical in vivo imaging technical note for IVIS Imaging Systems. Instrument background occurs when excitation light leaks through the emission filter. This occurs more frequently when the excitation and emission filters are narrowly separated. The ring you see is a result of non specific light reflecting off of the stage at an incident angle and passing through the filter causing what appears as leakage around the edges.

PDF 1 MB
AutoExposure

Auto-exposure technical note for IVIS pre-clinical imaging systems

PDF 1 MB
Background ROI

Subtracting Background ROI from a Sequence

PDF 1 MB
Determine Saturation

Determine Saturation for IVIS imaging systems - technical note

PDF 1 MB
Drawing ROIs

Technical notes for Drawing ROIs for IVIS in vivo imaging systems. The circle, square, free draw, or grid (for well plates) can be used to draw your ROIs. ROI selections,are user-specific and are dependent on the model being analyzed. It is irrelevant which shape that is used for a particular ROI.

PDF 669 KB
High Resolution Images

Acquisition of High Resolution Images. This quick reference guide is for those researchers who wish to perform analysis that requires high resolution including in vitro studies when one may want to discern aspects about cell layers, ex vivo tissue imaging, or imaging of tissue slices. You will not need this resolution in most in vivo studies.

PDF 1 MB
Image Overlay 2D

Not only is it possible to load multiple images as a group, for example multiple days of a longitudinal study, but it is also possible to load multiple images and Overlay them i.e. bioluminescent tumor with fluorescent targeted drug acquired in two separate images.

PDF 472 KB
Loading Groups of Images

For many studies, it may be desirable to open a group of images together, for example, analyzing multiple days of longitudinal study side by side using the same scale.

PDF 751 KB
Subject ROI

Subject ROI using IVIS imaging systems

PDF 2 MB
Working with Image Math

Working with Image Math. Image Math is a rudimentary but effective method for Spectrum and Lumina users to subtract background from images without performing Spectral Unmixing.

PDF 911 KB

Video Article

Webinars

White Paper

Non-Invasive Optical Imaging for Viral Research and Novel Therapeutic and Vaccine Development

Viral diseases have emerged and re-emerged throughout history, and as the human population continues to increase globally, so will the frequency of viral pandemics. Not only have Ebola and COVID-19 demonstrated most recently mankind’s vulnerability to contagious diseases, but also the challenges we are faced with from a therapeutic standpoint.

Read how non-invasive optical imaging enables the most intricate host-pathogen interactions to be visualized and monitored in disease models that mimic what is seen in humans. Not only does optical imaging play an important role in better understanding the complex mechanisms of viral biology, it plays a vital role in the discovery and development of new drug and vaccine candidates.

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The Role of In Vivo Imaging in Drug Discovery and Development

The primary goal of preclinical imaging is to improve the odds of clinical success and reduce drug discovery and development time and costs. Advances in non-invasive in vivo imaging techniques have raised the use of animal models in drug discovery and development to a new level by enabling quick and efficient drug screening and evaluation. Read this White Paper to learn how preclinical in vivo imaging helps to ensure that smart choices are made by providing Go/No-Go decisions and de-risking drug candidates early on, significantly reducing time to the clinic and lowering costs all while maximizing biological understanding.

PDF 547 KB